Open Access

Exosomal connexin 43 regulates the resistance of glioma cells to temozolomide

  • Authors:
    • Zhang-Jian Yang
    • Le-Ling Zhang
    • Qiu-Chen Bi
    • Li-Jun Gan
    • Min-Jun Wei
    • Tao Hong
    • Ren-Jie Tan
    • Xue-Mei Lan
    • Li-Hua Liu
    • Xiao-Jian Han
    • Li-Ping Jiang
  • View Affiliations

  • Published online on: February 25, 2021     https://doi.org/10.3892/or.2021.7995
  • Article Number: 44
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Glioblastoma is the most common and aggressive brain tumor and it is characterized by a high mortality rate. Temozolomide (TMZ) is an effective chemotherapy drug for glioblastoma, but the resistance to TMZ has come to represent a major clinical problem, and its underlying mechanism has yet to be elucidated. In the present study, the role of exosomal connexin 43 (Cx43) in the resistance of glioma cells to TMZ and cell migration was investigated. First, higher expression levels of Cx43 were detected in TMZ‑resistant U251 (U251r) cells compared with those in TMZ‑sensitive (U251s) cells. Exosomes from U251s or U251r cells (sExo and rExo, respectively) were isolated. It was found that the expression of Cx43 in rExo was notably higher compared with that in sExo, whereas treatment with rExo increased the expression of Cx43 in U251s cells. Additionally, exosomes stained with dioctadecyloxacarbocyanine (Dio) were used to visualized exosome uptake by glioma cells. It was observed that the uptake of Dio‑stained rExo in U251s cells was more prominent compared with that of Dio‑stained sExo, while 37,43Gap27, a gap junction mimetic peptide directed against Cx43, alleviated the rExo uptake by cells. Moreover, rExo increased the IC50 of U251s to TMZ, colony formation and Bcl‑2 expression, but decreased Bax and cleaved caspase‑3 expression in U251s cells. 37,43Gap27 efficiently inhibited these effects of rExo on U251s cells. Finally, the results of the wound healing and Transwell assays revealed that rExo significantly enhanced the migration of U251s cells, whereas 37,43Gap27 significantly attenuated rExo‑induced cell migration. Taken together, these results indicate the crucial role of exosomal Cx43 in chemotherapy resistance and migration of glioma cells, and suggest that Cx43 may hold promise as a therapeutic target for glioblastoma in the future.
View Figures
View References

Related Articles

Journal Cover

April-2021
Volume 45 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yang Z, Zhang L, Bi Q, Gan L, Wei M, Hong T, Tan R, Lan X, Liu L, Han X, Han X, et al: Exosomal connexin 43 regulates the resistance of glioma cells to temozolomide. Oncol Rep 45: 44, 2021
APA
Yang, Z., Zhang, L., Bi, Q., Gan, L., Wei, M., Hong, T. ... Jiang, L. (2021). Exosomal connexin 43 regulates the resistance of glioma cells to temozolomide. Oncology Reports, 45, 44. https://doi.org/10.3892/or.2021.7995
MLA
Yang, Z., Zhang, L., Bi, Q., Gan, L., Wei, M., Hong, T., Tan, R., Lan, X., Liu, L., Han, X., Jiang, L."Exosomal connexin 43 regulates the resistance of glioma cells to temozolomide". Oncology Reports 45.4 (2021): 44.
Chicago
Yang, Z., Zhang, L., Bi, Q., Gan, L., Wei, M., Hong, T., Tan, R., Lan, X., Liu, L., Han, X., Jiang, L."Exosomal connexin 43 regulates the resistance of glioma cells to temozolomide". Oncology Reports 45, no. 4 (2021): 44. https://doi.org/10.3892/or.2021.7995