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Sodium bicarbonate transporter NBCe1 regulates proliferation and viability of human prostate cancer cells LNCaP and PC3

  • Authors:
    • Jun Ming Li
    • Soojung Lee
    • Reda Zafar
    • Eunjung Shin
    • Inyeong Choi
  • View Affiliations / Copyright

    Affiliations: Department of Physiology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 129
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    Published online on: May 17, 2021
       https://doi.org/10.3892/or.2021.8080
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Abstract

Studies on cultured cancer cells or cell lines have revealed multiple acid extrusion mechanisms and their involvement in cancer cell growth and progression. In the present study, the role of the sodium bicarbonate transporters (NBCs) in prostate cancer cell proliferation and viability was examined. qPCR revealed heterogeneous expression of five NBC isoforms in human prostate cancer cell lines LNCaP, PC3, 22RV1, C4-2, DU145, and the prostate cell line RWPE-1. In fluorescence pH measurement of LNCaP cells, which predominantly express NBCe1, Na+ and HCO3–-mediated acid extrusion was identified by bath ion replacement and sensitivity to the NBC inhibitor S0859. NBCe1 knockdown using siRNA oligonucleotides decreased the number of viable cells, and pharmacological inhibition with S0859 (50 µM) resulted in a similar decrease. NBCe1 knockdown and inhibition also increased cell death, but this effect was small and slow. In PC3 cells, which express all NBC isoforms, NBCe1 knockdown decreased viable cell number and increased cell death. The effects of NBCe1 knockdown were comparable to those by S0859, indicating that NBCe1 among NBCs primarily contributes to PC3 cell proliferation and viability. S0859 inhibition also decreased the formation of cell spheres in 3D cultures. Immunohistochemistry of human prostate cancer tissue microarrays revealed NBCe1 localization to the glandular epithelial cells in prostate tissue and robust expression in acinar and duct adenocarcinoma. In conclusion, our study demonstrates that NBCe1 regulates acid extrusion in prostate cancer cells and inhibiting or abolishing this transporter decreases cancer cell proliferation.
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Copy and paste a formatted citation
Spandidos Publications style
Li JM, Lee S, Zafar R, Shin E and Choi I: Sodium bicarbonate transporter NBCe1 regulates proliferation and viability of human prostate cancer cells LNCaP and PC3. Oncol Rep 46: 129, 2021.
APA
Li, J.M., Lee, S., Zafar, R., Shin, E., & Choi, I. (2021). Sodium bicarbonate transporter NBCe1 regulates proliferation and viability of human prostate cancer cells LNCaP and PC3. Oncology Reports, 46, 129. https://doi.org/10.3892/or.2021.8080
MLA
Li, J. M., Lee, S., Zafar, R., Shin, E., Choi, I."Sodium bicarbonate transporter NBCe1 regulates proliferation and viability of human prostate cancer cells LNCaP and PC3". Oncology Reports 46.1 (2021): 129.
Chicago
Li, J. M., Lee, S., Zafar, R., Shin, E., Choi, I."Sodium bicarbonate transporter NBCe1 regulates proliferation and viability of human prostate cancer cells LNCaP and PC3". Oncology Reports 46, no. 1 (2021): 129. https://doi.org/10.3892/or.2021.8080
Copy and paste a formatted citation
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Spandidos Publications style
Li JM, Lee S, Zafar R, Shin E and Choi I: Sodium bicarbonate transporter NBCe1 regulates proliferation and viability of human prostate cancer cells LNCaP and PC3. Oncol Rep 46: 129, 2021.
APA
Li, J.M., Lee, S., Zafar, R., Shin, E., & Choi, I. (2021). Sodium bicarbonate transporter NBCe1 regulates proliferation and viability of human prostate cancer cells LNCaP and PC3. Oncology Reports, 46, 129. https://doi.org/10.3892/or.2021.8080
MLA
Li, J. M., Lee, S., Zafar, R., Shin, E., Choi, I."Sodium bicarbonate transporter NBCe1 regulates proliferation and viability of human prostate cancer cells LNCaP and PC3". Oncology Reports 46.1 (2021): 129.
Chicago
Li, J. M., Lee, S., Zafar, R., Shin, E., Choi, I."Sodium bicarbonate transporter NBCe1 regulates proliferation and viability of human prostate cancer cells LNCaP and PC3". Oncology Reports 46, no. 1 (2021): 129. https://doi.org/10.3892/or.2021.8080
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