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Article

Egr‑1 inhibits colon cancer cell proliferation, migration and invasion via regulating CDKL1 at the transcriptional level

  • Authors:
    • Shanshan Shao
    • Man Ju
    • Jiayun Lei
    • Xiangqian Lu
    • Hongzhi Li
    • Darui Wang
    • Chaofeng Xia
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The Hospital Affiliated to Medical School of Yangzhou University (Taizhou People's Hospital), Taizhou, Jiangsu 225300, P.R. China, Department of Anus and Intestine Surgery, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China, Department of Oncology, Liaocheng Dongchangfu People's Hospital, Liaocheng, Shandong 252000, P.R. China, Department of Radiotherapy, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China, Department of Clinical Laboratory, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China
  • Article Number: 169
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    Published online on: June 23, 2021
       https://doi.org/10.3892/or.2021.8120
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Abstract

Colon cancer is one of the most common malignant tumors worldwide, and the molecular mechanisms involved in the oncogenesis and progression of colon cancer remain unclear. Early growth response 1 (Egr‑1) is a transcription factor that is closely associated with several tumor processes; however, its role in colon cancer is unknown. The present study aimed to explore the function and mechanism of transcription factor Egr‑1 in colon cancer progression. The association between Egr‑1 expression and the survival of patients with colon cancer was analyzed. Transwell assay was used to measure the migration and invasion of colon cancer cells. Cell Counting Kit‑8 assay was used to evaluate the cell proliferative ability. Reverse transcription‑quantitative PCR and western blot assays were used to identify whether Egr‑1 could regulate cyclin‑dependent kinase‑like 1 (CDKL1). Luciferase and chromatin immunoprecipitation assays were used to detect the mechanism by which Egr‑1 regulated CDKL1. Based on The Cancer Genome Atlas database, it was found that low Egr‑1 expression was associated with a poor prognosis in patients with colon cancer. Furthermore, overexpression of Egr‑1 inhibited colon cancer cell proliferation, migration, and invasion, whereas knockdown of Egr‑1 increased colon cancer cell proliferation, migration and invasion. Additionally, overexpression of Egr‑1‑induced cell proliferation, migration and invasion were reversed by overexpression of CDKL1. Furthermore, it was demonstrated that Egr‑1 regulated CDKL1 expression at the transcriptional level. The present study illustrated the mechanism of Egr‑1 regulating CDKL1, by which Egr‑1 affected colon cancer cell proliferation, migration and invasion. The current findings suggested that Egr‑1/CDKL1 may be a new promising target for the treatment of colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Shao S, Ju M, Lei J, Lu X, Li H, Wang D and Xia C: Egr‑1 inhibits colon cancer cell proliferation, migration and invasion via regulating CDKL1 at the transcriptional level. Oncol Rep 46: 169, 2021.
APA
Shao, S., Ju, M., Lei, J., Lu, X., Li, H., Wang, D., & Xia, C. (2021). Egr‑1 inhibits colon cancer cell proliferation, migration and invasion via regulating CDKL1 at the transcriptional level. Oncology Reports, 46, 169. https://doi.org/10.3892/or.2021.8120
MLA
Shao, S., Ju, M., Lei, J., Lu, X., Li, H., Wang, D., Xia, C."Egr‑1 inhibits colon cancer cell proliferation, migration and invasion via regulating CDKL1 at the transcriptional level". Oncology Reports 46.2 (2021): 169.
Chicago
Shao, S., Ju, M., Lei, J., Lu, X., Li, H., Wang, D., Xia, C."Egr‑1 inhibits colon cancer cell proliferation, migration and invasion via regulating CDKL1 at the transcriptional level". Oncology Reports 46, no. 2 (2021): 169. https://doi.org/10.3892/or.2021.8120
Copy and paste a formatted citation
x
Spandidos Publications style
Shao S, Ju M, Lei J, Lu X, Li H, Wang D and Xia C: Egr‑1 inhibits colon cancer cell proliferation, migration and invasion via regulating CDKL1 at the transcriptional level. Oncol Rep 46: 169, 2021.
APA
Shao, S., Ju, M., Lei, J., Lu, X., Li, H., Wang, D., & Xia, C. (2021). Egr‑1 inhibits colon cancer cell proliferation, migration and invasion via regulating CDKL1 at the transcriptional level. Oncology Reports, 46, 169. https://doi.org/10.3892/or.2021.8120
MLA
Shao, S., Ju, M., Lei, J., Lu, X., Li, H., Wang, D., Xia, C."Egr‑1 inhibits colon cancer cell proliferation, migration and invasion via regulating CDKL1 at the transcriptional level". Oncology Reports 46.2 (2021): 169.
Chicago
Shao, S., Ju, M., Lei, J., Lu, X., Li, H., Wang, D., Xia, C."Egr‑1 inhibits colon cancer cell proliferation, migration and invasion via regulating CDKL1 at the transcriptional level". Oncology Reports 46, no. 2 (2021): 169. https://doi.org/10.3892/or.2021.8120
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