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lncRNA TINCR attenuates the proliferation and invasion, and enhances the apoptosis of cutaneous malignant melanoma cells by regulating the miR‑424‑5p/LATS1 axis

  • Authors:
    • Xuemei Han
    • Yuxi Jia
    • Xiangru Chen
    • Chengkuan Sun
    • Jing Sun
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, The China‑Japan Union Hospital of Ji Lin University, Changchun, Jilin 130033, P.R. China, Department of Dermatology, The China‑Japan Union Hospital of Ji Lin University, Changchun, Jilin 130033, P.R. China, Department of Handsurgery, The China‑Japan Union Hospital of Ji Lin University, Changchun, Jilin 130033, P.R. China
    Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 238
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    Published online on: September 20, 2021
       https://doi.org/10.3892/or.2021.8189
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Abstract

Cutaneous malignant melanoma (CMM) is responsible for ≥1/2 of skin cancer‑related mortalities. The aberrant expression of long non‑coding RNAs (lncRNAs) has been associated with the development of CMM. However, to the best of our knowledge, the role of the lncRNA TINCR ubiquitin domain containing (TINCR) in CMM has not been previously investigated, and thus, the current study aimed to evaluate this in vitro and in vivo. Reverse transcription‑quantitative PCR (RT‑qPCR) was used to analyze microRNA (miR)‑424‑5p expression, and RT‑qPCR and western blotting were used to measure TINCR, large tumor suppressor kinase 1 (LATS1), cellular communication network factor 2 (CTGF), cellular communication network factor 1 (CCN1) and AXL receptor tyrosine kinase (AXL) mRNA and protein expression levels, respectively. Cell Counting Kit‑8, flow cytometry and Transwell assays were used to detect the proliferation, apoptosis and invasion of CMM cell lines, respectively. The binding sites between TINCR and miR‑424‑5p were predicted using the miRDB database. A dual luciferase reporter assay and RT‑qPCR were used to identify the relationship between TINCR and miR‑424‑5p in CMM cell lines. The bioinformatics analysis revealed that TINCR was one of the most significantly downregulated lncRNAs in CMM, and advanced stage CMM tissues showed the greatest decrease in TINCR expression. Moreover, in the collected CMM tissues and tested cell lines of the current study, TINCR expression was found to be downregulated compared with the respective controls. Notably, TINCR overexpression inhibited the expression levels of CTGF, CCN1 and AXL, decreased the proliferation and invasion, and induced the apoptosis of CMM cell lines. In addition, a mutual binding association was identified between miR‑424‑5p and TINCR in CMM cells. LATS1, a target of miR‑424‑5p, was found to be positively regulated by TINCR. TINCR activated Hippo signaling and repressed the activity of Yes 1 associated transcriptional regulator by regulating LATS1 expression, while LATS1 knockdown reversed the effect of TINCR overexpression on CMM cells. Collectively, the findings of the present study suggested that TINCR may attenuate the progression of CMM by regulating the miR‑424‑5p/LATS1 signaling axis. These results indicated that TINCR may play a tumor suppressive role in CMM.
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Copy and paste a formatted citation
Spandidos Publications style
Han X, Jia Y, Chen X, Sun C and Sun J: lncRNA TINCR attenuates the proliferation and invasion, and enhances the apoptosis of cutaneous malignant melanoma cells by regulating the miR‑424‑5p/LATS1 axis. Oncol Rep 46: 238, 2021.
APA
Han, X., Jia, Y., Chen, X., Sun, C., & Sun, J. (2021). lncRNA TINCR attenuates the proliferation and invasion, and enhances the apoptosis of cutaneous malignant melanoma cells by regulating the miR‑424‑5p/LATS1 axis. Oncology Reports, 46, 238. https://doi.org/10.3892/or.2021.8189
MLA
Han, X., Jia, Y., Chen, X., Sun, C., Sun, J."lncRNA TINCR attenuates the proliferation and invasion, and enhances the apoptosis of cutaneous malignant melanoma cells by regulating the miR‑424‑5p/LATS1 axis". Oncology Reports 46.5 (2021): 238.
Chicago
Han, X., Jia, Y., Chen, X., Sun, C., Sun, J."lncRNA TINCR attenuates the proliferation and invasion, and enhances the apoptosis of cutaneous malignant melanoma cells by regulating the miR‑424‑5p/LATS1 axis". Oncology Reports 46, no. 5 (2021): 238. https://doi.org/10.3892/or.2021.8189
Copy and paste a formatted citation
x
Spandidos Publications style
Han X, Jia Y, Chen X, Sun C and Sun J: lncRNA TINCR attenuates the proliferation and invasion, and enhances the apoptosis of cutaneous malignant melanoma cells by regulating the miR‑424‑5p/LATS1 axis. Oncol Rep 46: 238, 2021.
APA
Han, X., Jia, Y., Chen, X., Sun, C., & Sun, J. (2021). lncRNA TINCR attenuates the proliferation and invasion, and enhances the apoptosis of cutaneous malignant melanoma cells by regulating the miR‑424‑5p/LATS1 axis. Oncology Reports, 46, 238. https://doi.org/10.3892/or.2021.8189
MLA
Han, X., Jia, Y., Chen, X., Sun, C., Sun, J."lncRNA TINCR attenuates the proliferation and invasion, and enhances the apoptosis of cutaneous malignant melanoma cells by regulating the miR‑424‑5p/LATS1 axis". Oncology Reports 46.5 (2021): 238.
Chicago
Han, X., Jia, Y., Chen, X., Sun, C., Sun, J."lncRNA TINCR attenuates the proliferation and invasion, and enhances the apoptosis of cutaneous malignant melanoma cells by regulating the miR‑424‑5p/LATS1 axis". Oncology Reports 46, no. 5 (2021): 238. https://doi.org/10.3892/or.2021.8189
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