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Article

Characterization of morphological alterations in micropapillary adenocarcinoma of the lung using an established cell line

  • Authors:
    • Dai Sonoda
    • Koki Kamizaki
    • Yukiko Matsuo
    • Kana Aruga
    • Masashi Mikubo
    • Keishi Yamashita
    • Michiru Nishita
    • Yasuhiro Minami
    • Yukitoshi Satoh
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Kitasato University School of Medicine, Sagamihara, Kanagawa 252‑0374, Japan, Division of Cell Physiology, Department of Physiology and Cell Biology, Graduate School of Medicine, Kobe University, Kobe, Hyogo 650‑0017, Japan, Division of Advanced Surgical Oncology, Research and Development Center for New Medical Frontiers, Kitasato University School of Medicine, Sagamihara, Kanagawa 252‑0374, Japan, Department of Biochemistry, Fukushima Medical University School of Medicine, Fukushima, Fukushima 960‑1295, Japan
  • Article Number: 19
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    Published online on: November 19, 2021
       https://doi.org/10.3892/or.2021.8230
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Abstract

Micropapillary adenocarcinoma of the lung is a type of cancer associated with a poor prognosis and is characterized by the presence of tumor cells with a ring‑like glandular structure floating within alveolar spaces. In the present study, the association between its morphological, biochemical and immunohistochemical characteristics, and malignancy was investigated using the KU‑Lu‑MPPt3 cell line established from a patient with MIP adenocarcinoma. Two subpopulations of KU‑Lu‑MPPt3 cells, namely adhesive (AD) and clumpy and suspended (CS) cells, were prepared and subjected to DNA microarray, reverse transcription‑quantitative PCR, western blot and immunostaining analyses. Protein expression patterns were compared between the cell types and their derived tissues using immunostaining. The results revealed similar protein expression patterns between the tumor cells found in the alveolar spaces and CS cells, which exhibited morphological characteristic of MIP adenocarcinoma. Based on the results of DNA microarray analysis, the present study then focused on Akt and focal adhesion kinase (FAK), which were markedly activated in the KU‑Lu‑MPPt3 CS and AD cells, respectively. Following KU‑Lu‑MPPt3 CS cell plating onto collagen‑coated culture dishes, some cells exhibited a transformation of their morphology into KU‑Lu‑MPPt3 AD‑like cells within a few days, and their Akt and FAK activities were similar to those of the AD cells. Additionally, the inhibition of Akt and FAK activities with Akt and FAK inhibitors reduced KU‑Lu‑MPPt3 CS cell adhesion and proliferation. Thus, the aforementioned results indicated that the phosphorylation of FAK and Akt may play a crucial role in the regulation of KU‑Lu‑MPPt3 CS cell adhesion and proliferation, respectively. Furthermore, the malignant potential of MIP adenocarcinoma may be attributed to these morphological and biochemical alterations in the KU‑Lu‑MPPt3 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Sonoda D, Kamizaki K, Matsuo Y, Aruga K, Mikubo M, Yamashita K, Nishita M, Minami Y and Satoh Y: Characterization of morphological alterations in micropapillary adenocarcinoma of the lung using an established cell line. Oncol Rep 47: 19, 2022.
APA
Sonoda, D., Kamizaki, K., Matsuo, Y., Aruga, K., Mikubo, M., Yamashita, K. ... Satoh, Y. (2022). Characterization of morphological alterations in micropapillary adenocarcinoma of the lung using an established cell line. Oncology Reports, 47, 19. https://doi.org/10.3892/or.2021.8230
MLA
Sonoda, D., Kamizaki, K., Matsuo, Y., Aruga, K., Mikubo, M., Yamashita, K., Nishita, M., Minami, Y., Satoh, Y."Characterization of morphological alterations in micropapillary adenocarcinoma of the lung using an established cell line". Oncology Reports 47.1 (2022): 19.
Chicago
Sonoda, D., Kamizaki, K., Matsuo, Y., Aruga, K., Mikubo, M., Yamashita, K., Nishita, M., Minami, Y., Satoh, Y."Characterization of morphological alterations in micropapillary adenocarcinoma of the lung using an established cell line". Oncology Reports 47, no. 1 (2022): 19. https://doi.org/10.3892/or.2021.8230
Copy and paste a formatted citation
x
Spandidos Publications style
Sonoda D, Kamizaki K, Matsuo Y, Aruga K, Mikubo M, Yamashita K, Nishita M, Minami Y and Satoh Y: Characterization of morphological alterations in micropapillary adenocarcinoma of the lung using an established cell line. Oncol Rep 47: 19, 2022.
APA
Sonoda, D., Kamizaki, K., Matsuo, Y., Aruga, K., Mikubo, M., Yamashita, K. ... Satoh, Y. (2022). Characterization of morphological alterations in micropapillary adenocarcinoma of the lung using an established cell line. Oncology Reports, 47, 19. https://doi.org/10.3892/or.2021.8230
MLA
Sonoda, D., Kamizaki, K., Matsuo, Y., Aruga, K., Mikubo, M., Yamashita, K., Nishita, M., Minami, Y., Satoh, Y."Characterization of morphological alterations in micropapillary adenocarcinoma of the lung using an established cell line". Oncology Reports 47.1 (2022): 19.
Chicago
Sonoda, D., Kamizaki, K., Matsuo, Y., Aruga, K., Mikubo, M., Yamashita, K., Nishita, M., Minami, Y., Satoh, Y."Characterization of morphological alterations in micropapillary adenocarcinoma of the lung using an established cell line". Oncology Reports 47, no. 1 (2022): 19. https://doi.org/10.3892/or.2021.8230
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