Open Access

FGD5‑AS1 is an oncogenic lncRNA in pancreatic cancer and regulates the Wnt/β‑catenin signaling pathway via miR‑577

  • Authors:
    • Wei-Tao Zhang
    • Ji-Jun Zhang
    • Quan Shao
    • Ying-Kai Wang
    • Jie-Peng Jia
    • Bo Qian
    • Xiao-Wen Tian
    • Wen-Ji Yan
  • View Affiliations

  • Published online on: November 24, 2021     https://doi.org/10.3892/or.2021.8232
  • Article Number: 21
  • Copyright : © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

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Abstract

The objective of the present study was to clarify the expression characteristics of long non‑coding RNA (lncRNA) FGD5 antisense RNA 1 (FGD5‑AS1) in pancreatic cancer, as well as its biological function and underlying mechanism. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was utilized for the detection of FGD5‑AS1 and microRNA (miR)‑577 expression levels in pancreatic cancer tissues. Transfection was performed to upregulate or downregulate FGD5‑AS1 in pancreatic cancer cell lines. MTT and Transwell assays were then utilized to detect the proliferation, migration and invasion of cancer cells, respectively. Subsequently, dual‑luciferase reporter gene assay, RNA immunoprecipitation assay, RNA pull‑down assay, RT‑qPCR, western blotting, and Pearson's correlation analysis were employed to confirm the regulatory relationships among FGD5‑AS1, miR‑577, low‑density lipoprotein receptor‑related protein 6 (LRP6) and β‑catenin. Western blotting was employed to determine the expression levels of Axin2, cyclin D1 and c‑Myc. The expression level of FGD5‑AS1 was upregulated in pancreatic cancer tissues and cell lines. FGD5‑AS1 knockdown inhibited pancreatic cancer cell proliferation, migration and invasion. By contrast, miR‑577 was significantly inhibited in pancreatic cancer cells and tissues; its downregulation promoted pancreatic cancer cell proliferation, migration and invasion, and reversed the effects of FGD5‑AS1 knockdown on pancreatic cancer cells. In addition, it was revealed that miR‑577 was a target of FGD5‑AS1, and FGD5‑AS1 could modulate the expression levels of LRP6, β‑catenin, Axin2, cyclin D1 and c‑Myc via suppressing miR‑577. In conclusion, in pancreatic cancer, highly expressed FGD5‑AS1 activated the Wnt/β‑catenin signaling and promoted cancer cell proliferation, migration and invasion via suppression of miR‑577.
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January-2022
Volume 47 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhang W, Zhang J, Shao Q, Wang Y, Jia J, Qian B, Tian X and Yan W: FGD5‑AS1 is an oncogenic lncRNA in pancreatic cancer and regulates the Wnt/β‑catenin signaling pathway via miR‑577. Oncol Rep 47: 21, 2022
APA
Zhang, W., Zhang, J., Shao, Q., Wang, Y., Jia, J., Qian, B. ... Yan, W. (2022). FGD5‑AS1 is an oncogenic lncRNA in pancreatic cancer and regulates the Wnt/β‑catenin signaling pathway via miR‑577. Oncology Reports, 47, 21. https://doi.org/10.3892/or.2021.8232
MLA
Zhang, W., Zhang, J., Shao, Q., Wang, Y., Jia, J., Qian, B., Tian, X., Yan, W."FGD5‑AS1 is an oncogenic lncRNA in pancreatic cancer and regulates the Wnt/β‑catenin signaling pathway via miR‑577". Oncology Reports 47.1 (2022): 21.
Chicago
Zhang, W., Zhang, J., Shao, Q., Wang, Y., Jia, J., Qian, B., Tian, X., Yan, W."FGD5‑AS1 is an oncogenic lncRNA in pancreatic cancer and regulates the Wnt/β‑catenin signaling pathway via miR‑577". Oncology Reports 47, no. 1 (2022): 21. https://doi.org/10.3892/or.2021.8232