Systematic pan‑cancer analysis identifies CDC45 as having an oncogenic role in human cancers

Lu,Yalin; Chen,Xingjie; Liu,Fang; Yu,Hao; Zhang,Yu; Du,Kangjie; Nan,Yang; Huang,Qiang

doi:10.3892/or.2022.8400

Systematic pan‑cancer analysis identifies CDC45 as having an oncogenic role in human cancers

Authors:
- Yalin Lu
- Xingjie Chen
- Fang Liu
- Hao Yu
- Yu Zhang
- Kangjie Du
- Yang Nan
- Qiang Huang
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Affiliations: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China, Institute of Mental Health, Tianjin Anding Hospital, Mental Health Center of Tianjin Medical University, Tianjin 300052, P.R. China
Published online on: September 8, 2022 https://doi.org/10.3892/or.2022.8400
Article Number: 185
Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cell division cycle 45 (CDC45) is an essential protein required for the initiation of DNA replication. In the present study, the role of CDC45 across 33 cancers was systematically investigated. It was observed that the expression of CDC45 was significantly upregulated in most cancers, exhibiting a marked negative correlation with the overall survival. Next, there was no significant difference in prognosis between the genomically altered and unaltered groups with respect to clinical outcomes. A decreased level of CDC45 at the DNA promoter region was also identified in several cancers. Furthermore, CDC45 expression was associated with the levels of tumor‑infiltrating immune cells in some specific cancer types. In addition, CDC45 was associated with m⁶A methylation, and CDC45 expression was primarily positively correlated with ‘writers’ and ‘readers’ in various cancers, particularly HNRNPC, RBM15 and YTHDC1. Gene enrichment analysis was also performed. In addition, the AUC of each cancer with respect to its 1‑, 3‑, and 5‑year survival rates were explored. Finally, CCK‑8 assays, EdU assays and cell cycle analysis were conducted. In conclusion, the present study demonstrated that CDC45 may be a potential biomarker and target for cancer treatment.

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