Open Access

Promoting effects of calponin 3 on the growth of diffuse large B‑cell lymphoma cells

  • Authors:
    • Xiaojing Xing
    • Meichen Liu
    • Xuguang Wang
    • Qianxue Guo
    • Hongyue Wang
  • View Affiliations

  • Published online on: January 13, 2023     https://doi.org/10.3892/or.2023.8483
  • Article Number: 46
  • Copyright: © Xing et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Diffuse large B‑cell lymphoma (DLBCL) is one of the most common types of lymphoma. Calponin 3 (CNN3) is a thin filament‑associated protein previously known to regulate smooth muscle contraction. Recent evidence illustrates its involvement in carcinogenesis; however, its roles in DLBCL remain unknown. CNN3 was found to be highly expressed in DLBCL specimens according to the online Gene Expression Profiling Interactive Analysis data. The aim of the present study was to investigate the roles of CNN3 in the progression of DLBCL. In vitro, the ectopic expression of CNN3 promoted the proliferation and G1/S transition of DLBCL cells, while its silencing led to opposite alterations. A similar tumor‑promoting role of CNN3 was also demonstrated by injecting nude mice with DLBCL cells over‑ or underexpressing CNN3. The results of dual‑luciferase reporter and chromatin immunoprecipitation assays revealed that forkhead box O3 (FOXO3), a known tumor suppressor in DLBCL, bound to the CNN3 promoter at ‑1955/‑1948 and ‑1190/‑1183, and suppressed the transcription of CNN3. The alterations induced by FOXO3 were partly blocked by CNN3 overexpression. On the whole, the present study demonstrates that CNN3, whose transcriptional activity is negatively regulated by FOXO3, contributes to the malignant behavior of DLBCL cells. The findings of the present study may provide novel diagnostic or therapeutic insight for DLBCL in clinical practice.
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February-2023
Volume 49 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Xing X, Liu M, Wang X, Guo Q and Wang H: Promoting effects of calponin 3 on the growth of diffuse large B‑cell lymphoma cells. Oncol Rep 49: 46, 2023
APA
Xing, X., Liu, M., Wang, X., Guo, Q., & Wang, H. (2023). Promoting effects of calponin 3 on the growth of diffuse large B‑cell lymphoma cells. Oncology Reports, 49, 46. https://doi.org/10.3892/or.2023.8483
MLA
Xing, X., Liu, M., Wang, X., Guo, Q., Wang, H."Promoting effects of calponin 3 on the growth of diffuse large B‑cell lymphoma cells". Oncology Reports 49.2 (2023): 46.
Chicago
Xing, X., Liu, M., Wang, X., Guo, Q., Wang, H."Promoting effects of calponin 3 on the growth of diffuse large B‑cell lymphoma cells". Oncology Reports 49, no. 2 (2023): 46. https://doi.org/10.3892/or.2023.8483