Open Access

Andrographolide suppresses aerobic glycolysis and induces apoptotic cell death by inhibiting pyruvate dehydrogenase kinase 1 expression

  • Authors:
    • Eun-Sun Yang
    • Yunju Do
    • Se-Yun Cheon
    • Bosung Kim
    • Jin Ling
    • Min Kyoung Cho
    • Taekyung Kim
    • Sung-Jin Bae
    • Ki-Tae Ha
  • View Affiliations

  • Published online on: February 23, 2023     https://doi.org/10.3892/or.2023.8509
  • Article Number: 72
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Metabolic disorder is a major characteristic of cancer cells, and controlling genes involved in metabolic shifts can be an effective strategy for cancer treatment. Andrographolide (AG), a diterpenoid lactone, is widely recognized as a natural anticancer drug due to its ability to inhibit cancer growth. The present study aimed to investigate the mechanism underlying the mitochondrial‑mediated anticancer effect of AG by inhibiting pyruvate dehydrogenase kinase 1 (PDK1) expression in lung cancer cells. Cells were treated with AG and PDK1 mRNA and protein expression was determined using reverse transcription‑quantitative PCR and western blotting, respectively. As a result, AG significantly inhibited the viability of human lung cancer cells and suppressed aerobic glycolysis by decreasing lactate generation. AG further decreased the PDK1 protein and mRNA levels in a dose‑dependent manner. AG‑induced cell death was assessed by flow cytometry and fluorescence microscopy. AG induced apoptotic cell death that was associated with the cleavage of poly (ADP ribose) polymerase, activation of caspase‑3, and mitochondrial damage, which was associated with an increase in reactive oxygen species and loss of mitochondrial membrane potential. AG‑induced cell death was partially suppressed via PDK1 overexpression in lung cancer cells. Therefore, the anticancer effects of AG on human lung cancer cells may negatively regulate the expression of PDK1.
View Figures
View References

Related Articles

Journal Cover

April-2023
Volume 49 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yang E, Do Y, Cheon S, Kim B, Ling J, Cho MK, Kim T, Bae S and Ha K: Andrographolide suppresses aerobic glycolysis and induces apoptotic cell death by inhibiting pyruvate dehydrogenase kinase 1 expression. Oncol Rep 49: 72, 2023
APA
Yang, E., Do, Y., Cheon, S., Kim, B., Ling, J., Cho, M.K. ... Ha, K. (2023). Andrographolide suppresses aerobic glycolysis and induces apoptotic cell death by inhibiting pyruvate dehydrogenase kinase 1 expression. Oncology Reports, 49, 72. https://doi.org/10.3892/or.2023.8509
MLA
Yang, E., Do, Y., Cheon, S., Kim, B., Ling, J., Cho, M. K., Kim, T., Bae, S., Ha, K."Andrographolide suppresses aerobic glycolysis and induces apoptotic cell death by inhibiting pyruvate dehydrogenase kinase 1 expression". Oncology Reports 49.4 (2023): 72.
Chicago
Yang, E., Do, Y., Cheon, S., Kim, B., Ling, J., Cho, M. K., Kim, T., Bae, S., Ha, K."Andrographolide suppresses aerobic glycolysis and induces apoptotic cell death by inhibiting pyruvate dehydrogenase kinase 1 expression". Oncology Reports 49, no. 4 (2023): 72. https://doi.org/10.3892/or.2023.8509