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Nucleophosmin promotes lung adenocarcinoma cell proliferation, migration and invasion by activating the EGFR/MAPK signaling pathway

  • Authors:
    • Min Li
    • Rongrong Wu
    • Dongyi Zhu
    • Le Wang
    • Shinan Liu
    • Ruolan Wang
    • Chaowen Deng
    • Shenglin Zhang
    • Min Chen
    • Ruojin Lu
    • Hongxing Zhu
    • Mengting Mo
    • Zhuang Luo
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory and Critical Care Medicine, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China, Department of Radiology, The First People's Hospital of Yunnan Province (The Affiliated Hospital of Kunming University of Science and Technology), Kunming, Yunnan 650034, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 126
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    Published online on: May 5, 2023
       https://doi.org/10.3892/or.2023.8563
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Abstract

Lung adenocarcinoma (LUAD) is the main cause of death globally. The present study investigated the prognostic value and functional verification of nucleophosmin (NPM1) in LUAD. LUAD and normal samples from The Cancer Genome Atlas were analyzed to identify whether NPM1 is associated with LUAD prognosis. NPM1 protein expression level was verified by western blotting. Cell proliferation, migration and invasion were detected by Cell Counting Kit‑8, wound healing and Transwell assays, respectively. EGFR/MAPK pathway‑related proteins [phosphorylated (p)‑EGFR/EGFR, p‑MEK/MEK, and p‑ERK/ERK] expression was measured through western blotting. A xenograft tumor mice model was constructed to perform the in vivo verification. NPM1 was upregulated in LUAD cells, and high‑level NPM1 indicated poor prognosis in patients with LUAD. In vitro experiments revealed that NPM1 knockdown inhibited LUAD cell proliferation, migration and invasion. Moreover, protein expression of p‑EGFR/EGFR, p‑MEK/MEK and p‑ERK/ERK was reduced with the NPM1 silencing. Furthermore, EGF, an activator of the EGFR/MAPK pathway, reversed the effects of NPM1. In vivo experiments showed that NPM1 knockdown inhibited tumor growth and protein levels of p‑EGFR/EGFR, p‑MEK/MEK and p‑ERK/ERK. NPM1 is related to the poor prognosis of LUAD and promotes the malignant progression of LUAD by activating the EGFR/MAPK pathway. This discovery provides a new potential therapeutic target for the diagnosis and treatment of LUAD.
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Copy and paste a formatted citation
Spandidos Publications style
Li M, Wu R, Zhu D, Wang L, Liu S, Wang R, Deng C, Zhang S, Chen M, Lu R, Lu R, et al: Nucleophosmin promotes lung adenocarcinoma cell proliferation, migration and invasion by activating the EGFR/MAPK signaling pathway. Oncol Rep 49: 126, 2023.
APA
Li, M., Wu, R., Zhu, D., Wang, L., Liu, S., Wang, R. ... Luo, Z. (2023). Nucleophosmin promotes lung adenocarcinoma cell proliferation, migration and invasion by activating the EGFR/MAPK signaling pathway. Oncology Reports, 49, 126. https://doi.org/10.3892/or.2023.8563
MLA
Li, M., Wu, R., Zhu, D., Wang, L., Liu, S., Wang, R., Deng, C., Zhang, S., Chen, M., Lu, R., Zhu, H., Mo, M., Luo, Z."Nucleophosmin promotes lung adenocarcinoma cell proliferation, migration and invasion by activating the EGFR/MAPK signaling pathway". Oncology Reports 49.6 (2023): 126.
Chicago
Li, M., Wu, R., Zhu, D., Wang, L., Liu, S., Wang, R., Deng, C., Zhang, S., Chen, M., Lu, R., Zhu, H., Mo, M., Luo, Z."Nucleophosmin promotes lung adenocarcinoma cell proliferation, migration and invasion by activating the EGFR/MAPK signaling pathway". Oncology Reports 49, no. 6 (2023): 126. https://doi.org/10.3892/or.2023.8563
Copy and paste a formatted citation
x
Spandidos Publications style
Li M, Wu R, Zhu D, Wang L, Liu S, Wang R, Deng C, Zhang S, Chen M, Lu R, Lu R, et al: Nucleophosmin promotes lung adenocarcinoma cell proliferation, migration and invasion by activating the EGFR/MAPK signaling pathway. Oncol Rep 49: 126, 2023.
APA
Li, M., Wu, R., Zhu, D., Wang, L., Liu, S., Wang, R. ... Luo, Z. (2023). Nucleophosmin promotes lung adenocarcinoma cell proliferation, migration and invasion by activating the EGFR/MAPK signaling pathway. Oncology Reports, 49, 126. https://doi.org/10.3892/or.2023.8563
MLA
Li, M., Wu, R., Zhu, D., Wang, L., Liu, S., Wang, R., Deng, C., Zhang, S., Chen, M., Lu, R., Zhu, H., Mo, M., Luo, Z."Nucleophosmin promotes lung adenocarcinoma cell proliferation, migration and invasion by activating the EGFR/MAPK signaling pathway". Oncology Reports 49.6 (2023): 126.
Chicago
Li, M., Wu, R., Zhu, D., Wang, L., Liu, S., Wang, R., Deng, C., Zhang, S., Chen, M., Lu, R., Zhu, H., Mo, M., Luo, Z."Nucleophosmin promotes lung adenocarcinoma cell proliferation, migration and invasion by activating the EGFR/MAPK signaling pathway". Oncology Reports 49, no. 6 (2023): 126. https://doi.org/10.3892/or.2023.8563
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