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GOLPH3 inhibition overcomes cisplatin resistance by restoring the glutathione/reactive oxygen species balance in the A549 non‑small cell lung cancer cell line

  • Authors:
    • Qiongying Wei
    • Jinquan Lin
    • Zhuangbin Lin
    • Nanding Yu
    • Yingxiao Wu
    • Xuexue Tan
    • Dan Xue
  • View Affiliations / Copyright

    Affiliations: Department of Pulmonary and Critical Care Medicine, Fujian Medical University Union Hospital, Fuzhou, Fujian 350001, P.R. China, Department of Trauma Center and Emergency Surgery, The First Affiliated Hospital, Fujian Medical University, Fuzhou, Fujian 350005, P.R. China, Department of Radiation Oncology, Fujian Medical University Union Hospital, Fuzhou, Fujian 350001, P.R. China
    Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 170
    |
    Published online on: October 18, 2024
       https://doi.org/10.3892/or.2024.8829
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Abstract

Cisplatin resistance is common in non‑small cell lung cancer (NSCLC); however, the molecular mechanisms remain unclear. The present study aimed to identify a new function of Golgi phosphoprotein 3 (GOLPH3) in NSCLC‑associated cisplatin resistance. Using A549 human NSCLC cells and the cisplatin‑resistant variant, stable cell lines with GOLPH3 knockdown or overexpression were established using lentiviral vectors. Through Cell Counting Kit‑8 and EdU assays, it was revealed that knockdown of GOLPH3 significantly enhanced cisplatin sensitivity in NSCLC cells. Specifically, flow cytometric analysis showed that GOLPH3 knockdown promoted apoptosis and G2‑phase cell cycle arrest in A549 cells. Mechanistically, intracellular reactive oxygen species (ROS) and glutathione (GSH) levels were measured using assay kits, and it was demonstrated that GOLPH3 knockdown decreased intracellular GSH levels, and further attenuated intracellular cisplatin efflux and GSH/ROS imbalance. In addition, tumor‑sphere formation assays verified that GOLPH3 knockdown mitigated the stem cell‑like phenotype of NSCLC cells. In conclusion, the present findings indicated the relevance of GOLPH3 in NSCLC‑associated cisplatin resistance, and thus targeting GOLPH3 may be developed into a combination therapy to overcome cisplatin resistance.
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1 

Siegel RL, Miller KD, Wagle NS and Jemal A: Cancer statistics, 2023. CA Cancer J Clin. 73:17–48. 2023. View Article : Google Scholar : PubMed/NCBI

2 

Torre LA, Siegel RL and Jemal A: Lung cancer statistics. Adv Exp Med Biol. 893:1–19. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Miller KD, Nogueira L, Devasia T, Mariotto AB, Yabroff KR, Jemal A, Kramer J and Siegel RL: Cancer treatment and survivorship statistics, 2022. CA Cancer J Clin. 72:409–436. 2022. View Article : Google Scholar : PubMed/NCBI

4 

Wang M, Herbst RS and Boshoff C: Toward personalized treatment approaches for non-small-cell lung cancer. Nat Med. 27:1345–1356. 2021. View Article : Google Scholar : PubMed/NCBI

5 

Banfill K, Giuliani M, Aznar M, Franks K, McWilliam A, Schmitt M, Sun F, Vozenin MC and Faivre Finn C; IASLC Advanced Radiation Technology committee, : Cardiac toxicity of thoracic radiotherapy: Existing evidence and future directions. J Thorac Oncol. 16:216–227. 2021. View Article : Google Scholar : PubMed/NCBI

6 

Bentzen SM: Preventing or reducing late side effects of radiation therapy: Radiobiology meets molecular pathology. Nat Rev Cancer. 6:702–713. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Wennerberg E, Mukherjee S, Spada S, Hung C, Agrusa CJ, Chen C, Valeta-Magara A, Rudqvist NP, Van Nest SJ, Kamel MK, et al: Expression of the mono-ADP-ribosyltransferase ART1 by tumor cells mediates immune resistance in non-small cell lung cancer. Sci Transl Med. 14:eabe81952022. View Article : Google Scholar : PubMed/NCBI

8 

Horvath L, Thienpont B, Zhao L, Wolf D and Pircher A: Overcoming immunotherapy resistance in non-small cell lung cancer (NSCLC)-novel approaches and future outlook. Mol Cancer. 19:1412020. View Article : Google Scholar : PubMed/NCBI

9 

De Ruysscher D, Faivre-Finn C, Nackaerts K, Jordan K, Arends J, Douillard JY, Ricardi U and Peters S: Recommendation for supportive care in patients receiving concurrent chemotherapy and radiotherapy for lung cancer. Ann Oncol. 31:41–49. 2020. View Article : Google Scholar : PubMed/NCBI

10 

Rinaldi M, Cauchi C and Gridelli C: First line chemotherapy in advanced or metastatic NSCLC. Ann Oncol. 17 (Suppl 5):v64–v67. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Tsan DL, Lin CY, Kang CJ, Huang SF, Fan KH, Liao CT, Chen IH, Lee LY, Wang HM and Chang JT: The comparison between weekly and three-weekly cisplatin delivered concurrently with radiotherapy for patients with postoperative high-risk squamous cell carcinoma of the oral cavity. Radiat Oncol. 7:2152012. View Article : Google Scholar : PubMed/NCBI

12 

Chaft JE, Rimner A, Weder W, Azzoli CG, Kris MG and Cascone T: Evolution of systemic therapy for stages I–III non-metastatic non-small-cell lung cancer. Nat Rev Clin Oncol. 18:547–557. 2021. View Article : Google Scholar : PubMed/NCBI

13 

Pignon JP, Tribodet H, Scagliotti GV, Douillard JY, Shepherd FA, Stephens RJ, Dunant A, Torri V, Rosell R, Seymour L, et al: Lung adjuvant cisplatin evaluation: A pooled analysis by the LACE collaborative group. J Clin Oncol. 26:3552–3559. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Sun Y, Shen W, Hu S, Lyu Q, Wang Q, Wei T, Zhu W and Zhang J: METTL3 promotes chemoresistance in small cell lung cancer by inducing mitophagy. J Exp Clin Cancer Res. 42:652023. View Article : Google Scholar : PubMed/NCBI

15 

Wang D, Zhao C, Xu F, Zhang A, Jin M, Zhang K, Liu L, Hua Q, Zhao J, Liu J, et al: Cisplatin-resistant NSCLC cells induced by hypoxia transmit resistance to sensitive cells through exosomal PKM2. Theranostics. 11:2860–2875. 2021. View Article : Google Scholar : PubMed/NCBI

16 

Song JW, Zhu J, Wu XX, Tu T, Huang JQ, Chen GZ, Liang LY, Zhou CH, Xu X and Gong LY: GOLPH3/CKAP4 promotes metastasis and tumorigenicity by enhancing the secretion of exosomal WNT3A in non-small-cell lung cancer. Cell Death Dis. 12:9762021. View Article : Google Scholar : PubMed/NCBI

17 

Scott KL, Kabbarah O, Liang MC, Ivanova E, Anagnostou V, Wu J, Dhakal S, Wu M, Chen S, Feinberg T, et al: GOLPH3 modulates mTOR signalling and rapamycin sensitivity in cancer. Nature. 459:1085–1090. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Yu T, An Q, Cao XL, Yang H, Cui J, Li ZJ and Xiao G: GOLPH3 inhibition reverses oxaliplatin resistance of colon cancer cells via suppression of PI3K/AKT/mTOR pathway. Life Sci. 260:1182942020. View Article : Google Scholar : PubMed/NCBI

19 

Wang MZ, Qiu CZ, Yu WS, Guo YT, Wang CX and Chen ZX: GOLPH3 expression promotes the resistance of HT29 cells to 5-fluorouracil by activating multiple signaling pathways. Mol Med Rep. 17:542–548. 2018.PubMed/NCBI

20 

Gao Y, Yin Z, Qi Y, Peng H, Ma W, Wang R and Li W: Golgi phosphoprotein 3 promotes angiogenesis and sorafenib resistance in hepatocellular carcinoma via upregulating exosomal miR-494-3p. Cancer Cell Int. 22:352022. View Article : Google Scholar : PubMed/NCBI

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Meng F, Li Y, Liu Q, Sun L, Wang H, Li X, Li G and Chen F: Experimental study of camptothecin combined with drug-eluting bead transarterial chemoembolization in the rabbit VX2 liver tumor model. Front Oncol. 12:9069712022. View Article : Google Scholar : PubMed/NCBI

23 

Qi Y, Wei J and Zhang X: Requirement of transcription factor NME2 for the maintenance of the stemness of gastric cancer stem-like cells. Cell Death Dis. 12:9242021. View Article : Google Scholar : PubMed/NCBI

24 

Fuertes MA, Alonso C and Pérez JM: Biochemical modulation of Cisplatin mechanisms of action: Enhancement of antitumor activity and circumvention of drug resistance. Chem Rev. 103:645–662. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Zalewska-Ziob M, Adamek B, Kasperczyk J, Romuk E, Hudziec E, Chwalińska E, Dobija-Kubica K, Rogoziński P and Bruliński K: Activity of antioxidant enzymes in the tumor and adjacent noncancerous tissues of non-small-cell lung cancer. Oxid Med Cell Longev. 2019:29018402019. View Article : Google Scholar : PubMed/NCBI

26 

Wu C, Liu Z, Chen Z, Xu D, Chen L, Lin H and Shi J: A nonferrous ferroptosis-like strategy for antioxidant inhibition-synergized nanocatalytic tumor therapeutics. Sci Adv. 7:eabj88332021. View Article : Google Scholar : PubMed/NCBI

27 

Glasauer A, Sena LA, Diebold LP, Mazar AP and Chandel NS: Targeting SOD1 reduces experimental non-small-cell lung cancer. J Clin Invest. 124:117–128. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Karami Fath M, Ebrahimi M, Nourbakhsh E, Zia Hazara A, Mirzaei A, Shafieyari S, Salehi A, Hoseinzadeh M, Payandeh Z and Barati G: PI3K/Akt/mTOR signaling pathway in cancer stem cells. Pathol Res Pract. 237:1540102022. View Article : Google Scholar : PubMed/NCBI

29 

Yang L, Shi P, Zhao G, Xu J, Peng W, Zhang J, Zhang G, Wang X, Dong Z, Chen F and Cui H: Targeting cancer stem cell pathways for cancer therapy. Signal Transduct Target Ther. 5:82020. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wei Q, Lin J, Lin Z, Yu N, Wu Y, Tan X and Xue D: GOLPH3 inhibition overcomes cisplatin resistance by restoring the glutathione/reactive oxygen species balance in the A549 non‑small cell lung cancer cell line. Oncol Rep 52: 170, 2024.
APA
Wei, Q., Lin, J., Lin, Z., Yu, N., Wu, Y., Tan, X., & Xue, D. (2024). GOLPH3 inhibition overcomes cisplatin resistance by restoring the glutathione/reactive oxygen species balance in the A549 non‑small cell lung cancer cell line. Oncology Reports, 52, 170. https://doi.org/10.3892/or.2024.8829
MLA
Wei, Q., Lin, J., Lin, Z., Yu, N., Wu, Y., Tan, X., Xue, D."GOLPH3 inhibition overcomes cisplatin resistance by restoring the glutathione/reactive oxygen species balance in the A549 non‑small cell lung cancer cell line". Oncology Reports 52.6 (2024): 170.
Chicago
Wei, Q., Lin, J., Lin, Z., Yu, N., Wu, Y., Tan, X., Xue, D."GOLPH3 inhibition overcomes cisplatin resistance by restoring the glutathione/reactive oxygen species balance in the A549 non‑small cell lung cancer cell line". Oncology Reports 52, no. 6 (2024): 170. https://doi.org/10.3892/or.2024.8829
Copy and paste a formatted citation
x
Spandidos Publications style
Wei Q, Lin J, Lin Z, Yu N, Wu Y, Tan X and Xue D: GOLPH3 inhibition overcomes cisplatin resistance by restoring the glutathione/reactive oxygen species balance in the A549 non‑small cell lung cancer cell line. Oncol Rep 52: 170, 2024.
APA
Wei, Q., Lin, J., Lin, Z., Yu, N., Wu, Y., Tan, X., & Xue, D. (2024). GOLPH3 inhibition overcomes cisplatin resistance by restoring the glutathione/reactive oxygen species balance in the A549 non‑small cell lung cancer cell line. Oncology Reports, 52, 170. https://doi.org/10.3892/or.2024.8829
MLA
Wei, Q., Lin, J., Lin, Z., Yu, N., Wu, Y., Tan, X., Xue, D."GOLPH3 inhibition overcomes cisplatin resistance by restoring the glutathione/reactive oxygen species balance in the A549 non‑small cell lung cancer cell line". Oncology Reports 52.6 (2024): 170.
Chicago
Wei, Q., Lin, J., Lin, Z., Yu, N., Wu, Y., Tan, X., Xue, D."GOLPH3 inhibition overcomes cisplatin resistance by restoring the glutathione/reactive oxygen species balance in the A549 non‑small cell lung cancer cell line". Oncology Reports 52, no. 6 (2024): 170. https://doi.org/10.3892/or.2024.8829
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