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Detection of UV-induced K-ras codon 12 mutation by PCR and differential dot-blot hybridization in cells from Down syndrome and Cockayne syndrome.

  • Authors:
    • K Sugita
    • N Suzuki
    • K Ohno
    • Y Suzuki
    • J I Takanashi
    • K Kita
    • H Yamamori
    • N Nakajima

  • View Affiliations

  • Published online on: January 1, 1999     https://doi.org/10.3892/or.6.1.145
  • Pages: 145-152
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Abstract

By means of the polymerase chain reaction (PCR) and differential dot-blot hybridization, base substitution mutations of K-ras codon 12 were investigated in skin fibroblast cells from Down syndrome (DS) patients. Mutations were identified in DS cells after UV irradiation, predominantly in cells from younger patients. In contrast, no mutation was detected in cells from Cockayne syndrome (CS) patients who had the same features of premature aging as in DS but were not prone to cancer. This association of DS cells, but not CS cells, with inducibility of the K-ras codon 12 mutation may imply the proneness of DS patients to cancer development but a lack of proneness of CS patients.

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Jan-Feb 1999
Volume 6 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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  • Impact Factor: 4.2
  • Ranked Oncology
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  • CiteScore: 8.4
  • CiteScore Rank: Q1: #68/366 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.786
  • SCImago Journal Rank (SJR): 0.812
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Spandidos Publications style
Sugita K, Suzuki N, Ohno K, Suzuki Y, Takanashi J, Kita K, Yamamori H and Nakajima N: Detection of UV-induced K-ras codon 12 mutation by PCR and differential dot-blot hybridization in cells from Down syndrome and Cockayne syndrome.. Oncol Rep 6: 145-152, 1999
APA
Sugita, K., Suzuki, N., Ohno, K., Suzuki, Y., Takanashi, J., Kita, K. ... Nakajima, N. (1999). Detection of UV-induced K-ras codon 12 mutation by PCR and differential dot-blot hybridization in cells from Down syndrome and Cockayne syndrome.. Oncology Reports, 6, 145-152. https://doi.org/10.3892/or.6.1.145
MLA
Sugita, K., Suzuki, N., Ohno, K., Suzuki, Y., Takanashi, J., Kita, K., Yamamori, H., Nakajima, N."Detection of UV-induced K-ras codon 12 mutation by PCR and differential dot-blot hybridization in cells from Down syndrome and Cockayne syndrome.". Oncology Reports 6.1 (1999): 145-152.
Chicago
Sugita, K., Suzuki, N., Ohno, K., Suzuki, Y., Takanashi, J., Kita, K., Yamamori, H., Nakajima, N."Detection of UV-induced K-ras codon 12 mutation by PCR and differential dot-blot hybridization in cells from Down syndrome and Cockayne syndrome.". Oncology Reports 6, no. 1 (1999): 145-152. https://doi.org/10.3892/or.6.1.145