In vivo enhancement of chemosensitivity of human salivary gland cancer cells by overexpression of TGF-beta stimulated clone-22.

  • Authors:
    • F Omotehara
    • D Uchida
    • S Hino
    • N M Begum
    • H Yoshida
    • M Sato
    • H Kawamata
  • View Affiliations

  • Published online on: July 1, 2000     https://doi.org/10.3892/or.7.4.737
  • Pages: 737-777
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Abstract

We have isolated transforming growth factor-beta-stimulated clone-22 (TSC-22) cDNA as an anti-cancer drug-inducible gene in a human salivary gland cancer cell line, TYS. We have previously reported that TSC-22 negatively regulates the growth of TYS cells, and that overexpression of TSC-22 protein in TYS cells enhanced the in vitro chemosensitivity of the cells. In this study, we examined the in vivo chemosensitivity of TSC-22-expressing TYS cells. TSC-22-expressing TYS cells formed tumors in nude mice, but tumors formed by TSC-22-expressing TYS cells were significantly smaller than tumors formed by control cells (p<0.001, one way ANOVA). Furthermore, intraperitoneal injection of 5-fluorouracil (5-FU) markedly inhibited the growth of the TSC-22-expressing TYS tumors, but did not affect the growth of control tumors. It was found by TUNEL assay that TSC-22-expressing TYS tumors were induced to undergo apoptosis by 5-FU treatment. These findings suggest that overexpression of TSC-22 protein in TYS cells enhances the in vivo chemosensitivity of the cells to 5-FU via induction of apoptosis.

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Jul-Aug 2000
Volume 7 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Omotehara F, Uchida D, Hino S, Begum N, Yoshida H, Sato M and Kawamata H: In vivo enhancement of chemosensitivity of human salivary gland cancer cells by overexpression of TGF-beta stimulated clone-22.. Oncol Rep 7: 737-777, 2000
APA
Omotehara, F., Uchida, D., Hino, S., Begum, N., Yoshida, H., Sato, M., & Kawamata, H. (2000). In vivo enhancement of chemosensitivity of human salivary gland cancer cells by overexpression of TGF-beta stimulated clone-22.. Oncology Reports, 7, 737-777. https://doi.org/10.3892/or.7.4.737
MLA
Omotehara, F., Uchida, D., Hino, S., Begum, N., Yoshida, H., Sato, M., Kawamata, H."In vivo enhancement of chemosensitivity of human salivary gland cancer cells by overexpression of TGF-beta stimulated clone-22.". Oncology Reports 7.4 (2000): 737-777.
Chicago
Omotehara, F., Uchida, D., Hino, S., Begum, N., Yoshida, H., Sato, M., Kawamata, H."In vivo enhancement of chemosensitivity of human salivary gland cancer cells by overexpression of TGF-beta stimulated clone-22.". Oncology Reports 7, no. 4 (2000): 737-777. https://doi.org/10.3892/or.7.4.737