Establishment of new multidrug-resistant human osteosarcoma cell lines.

  • Authors:
    • Y Oda
    • Y Matsumoto
    • K Harimaya
    • Y Iwamoto
    • M Tsuneyoshi
  • View Affiliations

  • Published online on: July 1, 2000     https://doi.org/10.3892/or.7.4.859
  • Pages: 859-925
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Abstract

Multidrug-resistant clones of human osteosarcoma MNNG/HOS and MG63 cells were isolated by stepwise selection on exposure to increasing doses of doxorubicin (DXR). The final clones MNNG/HOS/DXR1000 and MG63/DXR1000, established after ethylmethane sulfonate mutagenesis, showed 96-fold and 121-fold higer resistance to DXR than their parental cell lines. They were also cross-resistant to vincristine, but not to cisplatinum or methotrexate. The levels of multidrug-resistance-1 (MDR1) mRNA expression increased gradually according to the concentration of DXR in both cell lines. Although the parental MNNG/HOS cells expressed a low level of MDR1 mRNA, the parental MG63 cells showed no MDR1 expression. The IC50 values of MNNG/HOS and its resistant variant to DXR were higher than those of MG63 and its resistant clone. Multidrug-resistant associated protein (MRP) mRNA expression was detected in MNNG/HOS or MG63 parental cell lines, and in their resistant variants. MG63 and its resistant variants revealed stable expression of MRP, whereas the resistant phenotype of MNNG/HOS showed decreased MRP expression, compared to its parental cell line. No alteration in the levels of hepatocyte growth factor (HGF) or its receptor c-MET was recognized between parental lines and their resistant variants. The results indicate that our DXR-resistant variants of MNNG/HOS and MG63 reveal a classical MDR phenotype and can offer a model with which to investigate the mechanisms of multidrug resistance in osteosarcoma.

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Jul-Aug 2000
Volume 7 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Oda Y, Matsumoto Y, Harimaya K, Iwamoto Y and Tsuneyoshi M: Establishment of new multidrug-resistant human osteosarcoma cell lines.. Oncol Rep 7: 859-925, 2000
APA
Oda, Y., Matsumoto, Y., Harimaya, K., Iwamoto, Y., & Tsuneyoshi, M. (2000). Establishment of new multidrug-resistant human osteosarcoma cell lines.. Oncology Reports, 7, 859-925. https://doi.org/10.3892/or.7.4.859
MLA
Oda, Y., Matsumoto, Y., Harimaya, K., Iwamoto, Y., Tsuneyoshi, M."Establishment of new multidrug-resistant human osteosarcoma cell lines.". Oncology Reports 7.4 (2000): 859-925.
Chicago
Oda, Y., Matsumoto, Y., Harimaya, K., Iwamoto, Y., Tsuneyoshi, M."Establishment of new multidrug-resistant human osteosarcoma cell lines.". Oncology Reports 7, no. 4 (2000): 859-925. https://doi.org/10.3892/or.7.4.859