Mitochondrial genotypes and radiation-induced micronucleus formation in human osteosarcoma cells in vitro

  • Authors:
    • Ken Yoshida
    • Hideya Yamazaki
    • Shuji Ozeki
    • Takehiro Inoue
    • Yasuo Yoshioka
    • Makoto Yoneda
    • Yoshisada Fujiwara
    • Toshihiko Inoue
  • View Affiliations

  • Published online on: May 1, 2001     https://doi.org/10.3892/or.8.3.615
  • Pages: 615-619
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Abstract

We investigated whether or not the mitochondrial genotypes affect radiation-induced micronucleus (MN) formation. For that purpose, the ρ+, KT1 and ρ0 human osteosarcoma cell lines were used, which carry the wild-type mitochondrial DNA (mtDNA), the tRNALys mutant mtDNA and no mtDNA, respectively. Despite no significant difference in the clonogenic radiosensitivity, the ρ+, KT1 and ρ0 cells exhibited high, intermediate and low radiosensitivities, respectively, to the MN induction in cytokinesis-blocked binucleated cells. Such differential MN inductions were correlated with high, intermediate and low levels of cellular ATP in the ρ+, KT1 and ρ0 cells, respectively, but not exactly with ROS production. Antimycin A that inhibits the respiratory complex III reduced the rate of radiation-induced MN induction in the ρ+ and KT1, but not ρ0 cells. Thus, the functional status of the mtDNA to produce ATP appears to play a significant role for radiation-induced MN.

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May-June 2001
Volume 8 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yoshida K, Yamazaki H, Ozeki S, Inoue T, Yoshioka Y, Yoneda M, Fujiwara Y and Inoue T: Mitochondrial genotypes and radiation-induced micronucleus formation in human osteosarcoma cells in vitro. Oncol Rep 8: 615-619, 2001
APA
Yoshida, K., Yamazaki, H., Ozeki, S., Inoue, T., Yoshioka, Y., Yoneda, M. ... Inoue, T. (2001). Mitochondrial genotypes and radiation-induced micronucleus formation in human osteosarcoma cells in vitro. Oncology Reports, 8, 615-619. https://doi.org/10.3892/or.8.3.615
MLA
Yoshida, K., Yamazaki, H., Ozeki, S., Inoue, T., Yoshioka, Y., Yoneda, M., Fujiwara, Y., Inoue, T."Mitochondrial genotypes and radiation-induced micronucleus formation in human osteosarcoma cells in vitro". Oncology Reports 8.3 (2001): 615-619.
Chicago
Yoshida, K., Yamazaki, H., Ozeki, S., Inoue, T., Yoshioka, Y., Yoneda, M., Fujiwara, Y., Inoue, T."Mitochondrial genotypes and radiation-induced micronucleus formation in human osteosarcoma cells in vitro". Oncology Reports 8, no. 3 (2001): 615-619. https://doi.org/10.3892/or.8.3.615