Diallyl sulfide inhibits PhIP-induced cell death via the inhibition of DNA strand breaks in normal human breast epithelial cells

  • Authors:
    • Ayoola Aboyade-Cole
    • Selina Darling-Reed
    • Ebenezer Oriaku
    • Michael McCaskill
    • Ronald Thomas
  • View Affiliations

  • Published online on: August 1, 2008     https://doi.org/10.3892/or_00000009
  • Pages: 319-323
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is associated with mammary carcinomas in animals and humans. PhIP is metabolized by CYP 1A1/1A2 and cytochrome b5 reductase, producing free radicals causing DNA strand breaks. Diallyl sulfide (DAS) prevents cancer in animals. We hypothesized that DAS will attenuate PhIP-induced DNA strand breaks and cell death. To test this hypothesis, we treated MCF-10A cells with PhIP, DAS and PhIP/DAS for 24, 48 and 72 h. DAS inhibited the PhIP-induced DNA strand breaks by 22% after 48 h and the strand breaks were completely inhibited at 72 h. PhIP reduced cell viability at each time point. However, DAS only attenuated this reduction in cell viability by 56% at 72 h. N-OH PhIP inhibited cell viability by 26% at 72 h. DAS completely attenuated this reduction in cell viability and may prevent PhIP-induced breast cancer via alterations in DNA damage and cell viability.

Related Articles

Journal Cover

August 2008
Volume 20 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Aboyade-Cole A, Darling-Reed S, Oriaku E, McCaskill M and Thomas R: Diallyl sulfide inhibits PhIP-induced cell death via the inhibition of DNA strand breaks in normal human breast epithelial cells. Oncol Rep 20: 319-323, 2008
APA
Aboyade-Cole, A., Darling-Reed, S., Oriaku, E., McCaskill, M., & Thomas, R. (2008). Diallyl sulfide inhibits PhIP-induced cell death via the inhibition of DNA strand breaks in normal human breast epithelial cells. Oncology Reports, 20, 319-323. https://doi.org/10.3892/or_00000009
MLA
Aboyade-Cole, A., Darling-Reed, S., Oriaku, E., McCaskill, M., Thomas, R."Diallyl sulfide inhibits PhIP-induced cell death via the inhibition of DNA strand breaks in normal human breast epithelial cells". Oncology Reports 20.2 (2008): 319-323.
Chicago
Aboyade-Cole, A., Darling-Reed, S., Oriaku, E., McCaskill, M., Thomas, R."Diallyl sulfide inhibits PhIP-induced cell death via the inhibition of DNA strand breaks in normal human breast epithelial cells". Oncology Reports 20, no. 2 (2008): 319-323. https://doi.org/10.3892/or_00000009