Antimycin A as a mitochondrial electron transport inhibitor prevents the growth of human lung cancer A549 cells

  • Authors:
    • Yong Hwan Han
    • Suhn Hee Kim
    • Sung Zoo Kim
    • Woo Hyun Park
  • View Affiliations

  • Published online on: September 1, 2008     https://doi.org/10.3892/or_00000061
  • Pages: 689-693
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Abstract

Antimycin A (AMA) inhibits mitochondrial electron transport between cytochromes b and c. We evaluated the effects of AMA on the growth of human pulmonary adenocarcinoma A549 cells in relation to cell cycle and apoptosis. Treatment with 2-100 µM AMA significantly inhibited the cell growth of A549 for 72 h. DNA flow cytometry indicated that AMA slightly induced a G1 phase arrest of the cell cycle for 72 h. Treatment with 50 µM AMA induced apoptosis of ≈17% in view of annexin V-staining cells. The dose of 50 µM AMA also induced loss of the mitochondrial membrane potential (ΔΨm) of ≈38%. The intracellular reactive oxygen species (ROS) levels including O2·−- were significantly increased in AMA-treated A549 cells. In conclusion, AMA inhibited the growth of A549 cells via inducing cell cycle arrest as well as triggering apoptosis. Growth inhibition in AMA-treated A549 cells was accompanied by an increase in ROS levels.

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September 2008
Volume 20 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Han YH, Kim SH, Kim SZ and Park WH: Antimycin A as a mitochondrial electron transport inhibitor prevents the growth of human lung cancer A549 cells. Oncol Rep 20: 689-693, 2008.
APA
Han, Y.H., Kim, S.H., Kim, S.Z., & Park, W.H. (2008). Antimycin A as a mitochondrial electron transport inhibitor prevents the growth of human lung cancer A549 cells. Oncology Reports, 20, 689-693. https://doi.org/10.3892/or_00000061
MLA
Han, Y. H., Kim, S. H., Kim, S. Z., Park, W. H."Antimycin A as a mitochondrial electron transport inhibitor prevents the growth of human lung cancer A549 cells". Oncology Reports 20.3 (2008): 689-693.
Chicago
Han, Y. H., Kim, S. H., Kim, S. Z., Park, W. H."Antimycin A as a mitochondrial electron transport inhibitor prevents the growth of human lung cancer A549 cells". Oncology Reports 20, no. 3 (2008): 689-693. https://doi.org/10.3892/or_00000061