The pro-angiogenic factors stimulated by human papillomavirus type 16 E6 and E7 protein in C33A and human fibroblasts

  • Authors:
    • Ling Xi
    • Shixuan Wang
    • Changyu Wang
    • Qian Xu
    • Pengcheng Li
    • Xun Tian
    • Peng Wu
    • Wei Wang
    • Dongrui Deng
    • Jianfeng Zhou
    • Ding Ma
  • View Affiliations

  • Published online on: January 1, 2009     https://doi.org/10.3892/or_00000185
  • Pages: 25-31
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Abstract

To investigate the pro-angiogenic factors stimulated by human papillomavirus type 16 E6 and E7 protein in C33A and human fibroblasts. HPV-16 E6 and E7 genes were transfected into C33A and HFB to detect the profiling of angiogenesis-associated factors with the TranSignal angiogenesis antibody array. The mRNA and protein levels of the cytokines were examined by traditional RT-PCR and Western blotting in both cell lines before and after transfection of HPV-16 E6 and E7. HPV-16 E6 and E7 genes were successfully transfected into C33A and HFB cells. On the sheet of antibody array, after transfection of HPV-16 E6 and E7, 6 other cytokines, Ang-1, FGFα, HGF, IL-6, IP-10 and PIGF besides VEGF, were detected at higher levels in C33A cells. Expression of 7 other cytokines besides IL-8, Ang-1, IL-1α, IL-1β, HGF, IL-6, VEGF and PIGF increased in HFB cells. The common cytokines in both cell lines were Ang-1, HGF, PIGF and VEGF. The mRNA and protein levels of the four cytokines were verified to increase by traditional RT-PCR and Western blotting in both cell lines after transfection of HPV-16 E6 and E7. Multiple pro-angiogenic cytokines could be stimulated by HPV-16 E6 and E7 protein both in cervical cancer cell line and normal human diploid cells. Anti-angiogenesis therapy may be effective alone or in combination with biologic means aimed at E6 and E7 in the treatment of cervical cancer.

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January 2009
Volume 21 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Xi L, Wang S, Wang C, Xu Q, Li P, Tian X, Wu P, Wang W, Deng D, Zhou J, Zhou J, et al: The pro-angiogenic factors stimulated by human papillomavirus type 16 E6 and E7 protein in C33A and human fibroblasts. Oncol Rep 21: 25-31, 2009.
APA
Xi, L., Wang, S., Wang, C., Xu, Q., Li, P., Tian, X. ... Ma, D. (2009). The pro-angiogenic factors stimulated by human papillomavirus type 16 E6 and E7 protein in C33A and human fibroblasts. Oncology Reports, 21, 25-31. https://doi.org/10.3892/or_00000185
MLA
Xi, L., Wang, S., Wang, C., Xu, Q., Li, P., Tian, X., Wu, P., Wang, W., Deng, D., Zhou, J., Ma, D."The pro-angiogenic factors stimulated by human papillomavirus type 16 E6 and E7 protein in C33A and human fibroblasts". Oncology Reports 21.1 (2009): 25-31.
Chicago
Xi, L., Wang, S., Wang, C., Xu, Q., Li, P., Tian, X., Wu, P., Wang, W., Deng, D., Zhou, J., Ma, D."The pro-angiogenic factors stimulated by human papillomavirus type 16 E6 and E7 protein in C33A and human fibroblasts". Oncology Reports 21, no. 1 (2009): 25-31. https://doi.org/10.3892/or_00000185