Tiron, a ROS scavenger, protects human lung cancer Calu-6 cells against antimycin A-induced cell death

  • Authors:
    • Yong Hwan Han
    • Woo Hyun Park
  • View Affiliations

  • Published online on: January 1, 2009     https://doi.org/10.3892/or_00000216
  • Pages: 253-261
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Antimycin A (AMA) inhibits the mitochondrial electron transport between cytochromes b and c. However, the relationship between AMA and lung cancer cells is poorly understood. In this study, we investigated the involvement of reactive oxygen species (ROS) and glutathione (GSH) in AMA-treated lung cancer Calu-6 cell death. Treatment with AMA reduced cell viability in a dose-dependent manner for 72 h. The intracellular ROS levels were decreased in Calu-6 cells treated with low doses of AMA (10, 25 or 50 µM) at 72 h. However, the levels increased in cells treated with a high dose of 100 µM AMA. Levels of O2·− were significantly increased in AMA-treated cells at 72 h. The increases in ROS levels including O2·− in AMA-treated cells were observed within 10 min. Treatment with AMA reduced the intracellular GSH content. SOD activity was up-regulated in AMA-treated Calu-6 cells at 72 h. However, catalase activity was down-regulated by AMA. Treatment with tiron, a ROS scavenger, reduced the intracellular ROS levels, which were associated with a partial reduction of apoptosis. Treatment with exogenous SOD and catalase significantly inhibited loss of the mitochondrial transmembrane potential (ΔΨm) in AMA-treated Calu-6 cells. In conclusion, our results suggest that the changes of intracellular ROS and GSH affect apoptosis in AMA-treated Calu-6 cells.

Related Articles

Journal Cover

January 2009
Volume 21 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Han YH and Park WH: Tiron, a ROS scavenger, protects human lung cancer Calu-6 cells against antimycin A-induced cell death. Oncol Rep 21: 253-261, 2009.
APA
Han, Y.H., & Park, W.H. (2009). Tiron, a ROS scavenger, protects human lung cancer Calu-6 cells against antimycin A-induced cell death. Oncology Reports, 21, 253-261. https://doi.org/10.3892/or_00000216
MLA
Han, Y. H., Park, W. H."Tiron, a ROS scavenger, protects human lung cancer Calu-6 cells against antimycin A-induced cell death". Oncology Reports 21.1 (2009): 253-261.
Chicago
Han, Y. H., Park, W. H."Tiron, a ROS scavenger, protects human lung cancer Calu-6 cells against antimycin A-induced cell death". Oncology Reports 21, no. 1 (2009): 253-261. https://doi.org/10.3892/or_00000216