More than 45% of A549 and H446 cells are cancer initiating cells: Evidence from cloning and tumorigenic analyses

  • Authors:
    • Xiangjiao Meng
    • Xiuwen Wang
    • Yawei Wang
  • View Affiliations

  • Published online on: April 1, 2009     https://doi.org/10.3892/or_00000314
  • Pages: 995-1000
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Abstract

Cancer stem cells have been isolated from human lung cancer and cancer cell lines. This study was designed to investigate the fraction of cancer stem cells in the A549 and H446 cell lines by cloning and tumorigenic analyses. We found that >45% of A549 and H446 cells formed large clones that are able to generate subclones and subsequently give rise to xenograft tumors in the BALB/C-nude mouse. Both CD133+ and CD133− cells of A549 and H446 possessed stem cell characteristics and exposure to Hoechst 33342 affected the clonogenicity and proliferation of single A549 and H446 cell. Moreover, based on cell cycle phase distribution and growth curve, most A549 and H446 cells may not be stem cells, but they acquired stem cell characteristics when they were alone. Therefore, these cells that formed large clones should be called cancer initiating cells, not stem cells. Thus, we concluded that >45% of A549 and H446 cells were cancer initiating cells, although many of them were neither CD133+ cells nor side population cells.

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April 2009
Volume 21 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Meng X, Wang X and Wang Y: More than 45% of A549 and H446 cells are cancer initiating cells: Evidence from cloning and tumorigenic analyses. Oncol Rep 21: 995-1000, 2009
APA
Meng, X., Wang, X., & Wang, Y. (2009). More than 45% of A549 and H446 cells are cancer initiating cells: Evidence from cloning and tumorigenic analyses. Oncology Reports, 21, 995-1000. https://doi.org/10.3892/or_00000314
MLA
Meng, X., Wang, X., Wang, Y."More than 45% of A549 and H446 cells are cancer initiating cells: Evidence from cloning and tumorigenic analyses". Oncology Reports 21.4 (2009): 995-1000.
Chicago
Meng, X., Wang, X., Wang, Y."More than 45% of A549 and H446 cells are cancer initiating cells: Evidence from cloning and tumorigenic analyses". Oncology Reports 21, no. 4 (2009): 995-1000. https://doi.org/10.3892/or_00000314