Curcumin induces apoptosis through FAS and FADD, in caspase-3-dependent and -independent pathways in the N18 mouse-rat hybrid retina ganglion cells

  • Authors:
    • Hsu-Feng Lu
    • Kuang-Chi Lai
    • Shu-Chun Hsu
    • Hui-Ju Lin
    • Mei-Due Yang
    • Yuan-Liang Chen
    • Ming-Jen Fan
    • Jai-Sing Yang
    • Pi-Yun Cheng
    • Chao-Lin Kuo
    • Jing-Gung Chung
  • View Affiliations

  • Published online on: July 1, 2009     https://doi.org/10.3892/or_00000411
  • Pages: 97-104
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Abstract

Curcumin, a naturally occurring yellow pigment isolated from turmeric, is a well known antioxidant with broad spectrum of anti-tumor activities against many human cancer cells. In this study, curcumin-induced cytotoxic effect of mouse-rat hybrid retina ganglion cells (N18) were investigated. For determining cell viability, the trypan blue exclusion and flow cytometric analysis were used. The curcumin-caused cell cycle arrest in N18 cells was examined by flow cytometry. Curcumin affect on the production of reactive oxygen species and Ca2+ and on the decrease of the level of mitochondria membrane potential (ΔΨm) were also examined by flow cytometry. Curcumin-induced apoptosis was determined by DAPI staining and Western blotting was used for examining the apoptotic signaling proteins. Cell cycle analysis showed that G2/M phase arrest and sub-G1 occurs in N18 cells following 48 h exposure to curcumin. Curcumin also caused a marked increase in apoptosis, as characterized by DNA fragmentation (sub-G1 phase formation) and DAPI staining, and dysfunction of mitochondria, which was associated with the activation of caspase-8, -9 and -3. Curcumin also promoted the levels of Fas and FADD, Bax, cytochrome c release, but decreased the levels of Bcl-2 causing changes of ΔΨm. Curcumin also induced endoplasmic reticulum stress in N18 cells which was based on the changes of GADD153 and GRP78 and caused Ca2+ release. Curcumin induced apoptosis through the intrinsic pathway and caspase-3-dependent and -independent pathways in N18 cells.

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July 2009
Volume 22 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Lu H, Lai K, Hsu S, Lin H, Yang M, Chen Y, Fan M, Yang J, Cheng P, Kuo C, Kuo C, et al: Curcumin induces apoptosis through FAS and FADD, in caspase-3-dependent and -independent pathways in the N18 mouse-rat hybrid retina ganglion cells. Oncol Rep 22: 97-104, 2009.
APA
Lu, H., Lai, K., Hsu, S., Lin, H., Yang, M., Chen, Y. ... Chung, J. (2009). Curcumin induces apoptosis through FAS and FADD, in caspase-3-dependent and -independent pathways in the N18 mouse-rat hybrid retina ganglion cells. Oncology Reports, 22, 97-104. https://doi.org/10.3892/or_00000411
MLA
Lu, H., Lai, K., Hsu, S., Lin, H., Yang, M., Chen, Y., Fan, M., Yang, J., Cheng, P., Kuo, C., Chung, J."Curcumin induces apoptosis through FAS and FADD, in caspase-3-dependent and -independent pathways in the N18 mouse-rat hybrid retina ganglion cells". Oncology Reports 22.1 (2009): 97-104.
Chicago
Lu, H., Lai, K., Hsu, S., Lin, H., Yang, M., Chen, Y., Fan, M., Yang, J., Cheng, P., Kuo, C., Chung, J."Curcumin induces apoptosis through FAS and FADD, in caspase-3-dependent and -independent pathways in the N18 mouse-rat hybrid retina ganglion cells". Oncology Reports 22, no. 1 (2009): 97-104. https://doi.org/10.3892/or_00000411