Dual functional molecular imaging probe targeting CD20 with PET and optical imaging

  • Authors:
    • Pramila Paudyal
    • Bishnuhari Paudyal
    • Yasuhiko Iida
    • Noboru Oriuchi
    • Hirofumi Hanaoka
    • Hideyuki Tominaga
    • Tomohiro Ishikita
    • Hiroki Yoshioka
    • Tetsuya Higuchi
    • Keigo Endo
  • View Affiliations

  • Published online on: July 1, 2009     https://doi.org/10.3892/or_00000413
  • Pages: 115-119
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Abstract

The present study aimed to develop a monoclonal antibody (mAb)-based double functional probe for PET and near-infrared fluorescence (NIRF) targeting CD20 and to cross validate the targeting efficacy of this dual functional probe. NuB2, anti CD20 mAb was conjugated with Alexa Fluor 750 and 64Cu through DOTA chelator. PET and NIRF imaging was carried out at 30 min and 24 h post-injection with 64Cu-DOTA-NuB2-Alexa Fluor 750 in CD20 positive Raji lymphoma-bearing mice. Fluorescence intensity and radio-activity were studied by ex vivo biodistribution study at 30 min, 24 and 48 h after injection. Raji tumor showed significantly higher uptake of DOTA-NuB2-Alexa Fluor 750 than that of DOTA-Alexa Fluor 750 (p<0.05). Significant correlation was obtained between the organ-to-muscle ratios measured by the radioactivity and fluorescence intensity (p<2.2e-16, r=0.94). Our findings demonstrate the effectiveness and feasibility of preparing an effective mAb-based dual functional imaging agent for PET and NIRF targeting the CD20 expression in lymphoma.

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July 2009
Volume 22 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Paudyal P, Paudyal B, Iida Y, Oriuchi N, Hanaoka H, Tominaga H, Ishikita T, Yoshioka H, Higuchi T, Endo K, Endo K, et al: Dual functional molecular imaging probe targeting CD20 with PET and optical imaging. Oncol Rep 22: 115-119, 2009.
APA
Paudyal, P., Paudyal, B., Iida, Y., Oriuchi, N., Hanaoka, H., Tominaga, H. ... Endo, K. (2009). Dual functional molecular imaging probe targeting CD20 with PET and optical imaging. Oncology Reports, 22, 115-119. https://doi.org/10.3892/or_00000413
MLA
Paudyal, P., Paudyal, B., Iida, Y., Oriuchi, N., Hanaoka, H., Tominaga, H., Ishikita, T., Yoshioka, H., Higuchi, T., Endo, K."Dual functional molecular imaging probe targeting CD20 with PET and optical imaging". Oncology Reports 22.1 (2009): 115-119.
Chicago
Paudyal, P., Paudyal, B., Iida, Y., Oriuchi, N., Hanaoka, H., Tominaga, H., Ishikita, T., Yoshioka, H., Higuchi, T., Endo, K."Dual functional molecular imaging probe targeting CD20 with PET and optical imaging". Oncology Reports 22, no. 1 (2009): 115-119. https://doi.org/10.3892/or_00000413