Apogossypolone inhibits cell growth by inducing cell cycle arrest in U937 cells

  • Authors:
    • Jian Sun
    • Zhi-Ming Li
    • Zhe-Yu Hu
    • Zhao-Lei Zeng
    • Da-Jun Yang
    • Wen-Qi Jiang
  • View Affiliations

  • Published online on: July 1, 2009     https://doi.org/10.3892/or_00000424
  • Pages: 193-198
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Abstract

We examined the effect of apogossypolone (ApoG2), a new derivative from gossypol on cell cycle regulation in U937 human leukemic monocyte lymphoma cells in vitro. ApoG2 decreased the viability of U937 cells by inducing G1 arrest followed by apoptosis in a dose-dependent manner. The G0/G1 phase of the cell cycle is regulated by cyclin-dependent kinases (Cdk), cyclins and cyclin-dependent kinase inhibitors (Cdki). We show by western blot analysis, that the ApoG2-induced G1 arrest was mediated through the increased expression of Cdki proteins (p21cip1/waf1) with a simultaneous decrease in cdk2, cdk4, cyclin D1 and cyclin E expression. The induction of apoptosis after treatment with ApoG2 for 12, 24 and 48 h was demonstrated by flow cytometry analysis. ApoG2 also induced cytochrome c release and activation of caspase-3. To our knowledge, this is the first time that ApoG2 has been reported to potently inhibit the proliferation of human monocytic lymphoma U937 cells through G1 arrest. These findings suggest that ApoG2 may be a potential chemotherapeutic agent for the treatment of cancer.

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July 2009
Volume 22 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Sun J, Li Z, Hu Z, Zeng Z, Yang D and Jiang W: Apogossypolone inhibits cell growth by inducing cell cycle arrest in U937 cells. Oncol Rep 22: 193-198, 2009
APA
Sun, J., Li, Z., Hu, Z., Zeng, Z., Yang, D., & Jiang, W. (2009). Apogossypolone inhibits cell growth by inducing cell cycle arrest in U937 cells. Oncology Reports, 22, 193-198. https://doi.org/10.3892/or_00000424
MLA
Sun, J., Li, Z., Hu, Z., Zeng, Z., Yang, D., Jiang, W."Apogossypolone inhibits cell growth by inducing cell cycle arrest in U937 cells". Oncology Reports 22.1 (2009): 193-198.
Chicago
Sun, J., Li, Z., Hu, Z., Zeng, Z., Yang, D., Jiang, W."Apogossypolone inhibits cell growth by inducing cell cycle arrest in U937 cells". Oncology Reports 22, no. 1 (2009): 193-198. https://doi.org/10.3892/or_00000424