Identification of candidate epigenetic biomarkers for ovarian cancer detection

  • Authors:
    • Yi-Wen Huang
    • Rachel A. Jansen
    • Enrica Fabbri
    • Dustin Potter
    • Sandya Liyanarachchi
    • Michael W.Y. Chan
    • Joseph C. Liu
    • Anne P.G. Crijns
    • Robert Brown
    • Kenneth P. Nephew
    • Ate G.J. van der Zee
    • David E. Cohn
    • Pearlly S. Yan
    • Tim H.-M. Huang
    • Huey-Jen L. Lin
  • View Affiliations

  • Published online on: October 1, 2009     https://doi.org/10.3892/or_00000509
  • Pages: 853-861
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Abstract

Ovarian cancer ranks the most lethal among gynecologic neoplasms in women. To develop potential biomarkers for diagnosis, we have identified five novel genes (CYP39A1, GTF2A1, FOXD4L4, EBP, and HAAO) that are hypermethylated in ovarian tumors, compared with the non-malignant normal ovarian surface epithelia, using the quantitative methylation-specific polymerase chain reactions. Interestingly enough, multivariate Cox regression analysis has identified hypermethylation of CYP39A1 correlated with an increase rate of relapsing (P=0.032, hazard ratio >1). Concordant hypermethylation in at least three loci was observed in 50 out of 55 (91%) of ovarian tumors examined. The test sensitivity and specificity were assessed to be 96 and 67% for CYP39A1; 95 and 88% for GTF2A1; 93 and 67% for FOXD4L4; 81 and 67% for EBP; 89 and 82% for HAAO, respectively. Our data have identified, for the first time, GTF2A1 alone, or GTF2A1 plus HAAO are excellent candidate biomarkers for detecting this disease. Moreover, the known functions of these gene products further implicate dysregulated transcriptional control, cholesterol metabolism, or synthesis of quinolinic acids, may play important roles in attributing to ovarian neoplasm. Molecular therapies, by reversing the aberrant epigenomes using inhibitory agents or by abrogating the upstream signaling pathways that convey the epigenomic perturbations, may be developed into promising treatment regimens.

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October 2009
Volume 22 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Huang Y, Jansen RA, Fabbri E, Potter D, Liyanarachchi S, Chan MW, Liu JC, Crijns AP, Brown R, Nephew KP, Nephew KP, et al: Identification of candidate epigenetic biomarkers for ovarian cancer detection. Oncol Rep 22: 853-861, 2009
APA
Huang, Y., Jansen, R.A., Fabbri, E., Potter, D., Liyanarachchi, S., Chan, M.W. ... Lin, H.L. (2009). Identification of candidate epigenetic biomarkers for ovarian cancer detection. Oncology Reports, 22, 853-861. https://doi.org/10.3892/or_00000509
MLA
Huang, Y., Jansen, R. A., Fabbri, E., Potter, D., Liyanarachchi, S., Chan, M. W., Liu, J. C., Crijns, A. P., Brown, R., Nephew, K. P., van der Zee, A. G., Cohn, D. E., Yan, P. S., Huang, T. H., Lin, H. L."Identification of candidate epigenetic biomarkers for ovarian cancer detection". Oncology Reports 22.4 (2009): 853-861.
Chicago
Huang, Y., Jansen, R. A., Fabbri, E., Potter, D., Liyanarachchi, S., Chan, M. W., Liu, J. C., Crijns, A. P., Brown, R., Nephew, K. P., van der Zee, A. G., Cohn, D. E., Yan, P. S., Huang, T. H., Lin, H. L."Identification of candidate epigenetic biomarkers for ovarian cancer detection". Oncology Reports 22, no. 4 (2009): 853-861. https://doi.org/10.3892/or_00000509