Underexpression of PPARγ is associated with aneuploidy and lower differentiation of thyroid tumours of follicular origin

  • Authors:
    • Carla Espadinha
    • António E. Pinto
    • Valeriano Leite
  • View Affiliations

  • Published online on: October 1, 2009     https://doi.org/10.3892/or_00000516
  • Pages: 907-913
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Peroxisome proliferator-activated receptor γ (PPARγ) gene is a nuclear receptor that is involved in thyroid tumourigenesis. Recently, our group has shown that follicular carcinomas underexpressing PPARγ protein are more prone to develop distant metastases, to invade locally, to present poorly differentiated areas and to persist after surgery. Aneuploidy is also observed in some thyroid tumours, particularly in the more advanced cases. The aim of the present study was to investigate the association of PPARγ expression with the degree of differentiation and ploidy status of benign and malignant thyroid neoplasias. DNA cytometric studies, ploidy and S-phase fraction (SPF) determination, and quantitative RT-PCR analysis of molecular markers specific for thyroid follicular cells, namely Tg (thyroglobulin), TSHR (TSH receptor) and NIS (Na+/I− symporter) were compared between thyroid lesions with positive or negative PPARγ protein expression. We observed that PPARγ-negative tissues expressed lower levels of Tg mRNA [4.66x106 a.u. (arbitrary units) ± 1.49x106], and were more frequently aneuploid (36%), and presented higher SPF (3.1%±0.4) than PPARγ-positive samples (Tg mRNA = 2.54x107 a.u. ± 9.72x106, P=0.0006; aneuploidy=8%, P=0.0031; SPF=2.2%±0.2, P=0.0430). A similar trend was also observed for TSHR and NIS mRNA, although not reaching statistical significance. This study showed that underexpression of PPARγ is associated with poor tumour differentiation, aneuploidy and higher cell proliferative activity. Therapies designed to modulate expression of PPARγ may have an impact on the growth of thyroid neoplasias.

Related Articles

Journal Cover

October 2009
Volume 22 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Espadinha C, Pinto AE and Leite V: Underexpression of PPARγ is associated with aneuploidy and lower differentiation of thyroid tumours of follicular origin. Oncol Rep 22: 907-913, 2009
APA
Espadinha, C., Pinto, A.E., & Leite, V. (2009). Underexpression of PPARγ is associated with aneuploidy and lower differentiation of thyroid tumours of follicular origin. Oncology Reports, 22, 907-913. https://doi.org/10.3892/or_00000516
MLA
Espadinha, C., Pinto, A. E., Leite, V."Underexpression of PPARγ is associated with aneuploidy and lower differentiation of thyroid tumours of follicular origin". Oncology Reports 22.4 (2009): 907-913.
Chicago
Espadinha, C., Pinto, A. E., Leite, V."Underexpression of PPARγ is associated with aneuploidy and lower differentiation of thyroid tumours of follicular origin". Oncology Reports 22, no. 4 (2009): 907-913. https://doi.org/10.3892/or_00000516