The antitumor immune responses induced by nanoemulsion-encapsulated MAGE1-HSP70/SEA complex protein vaccine following different administration routes

  • Authors:
    • Wei Ge
    • Pei-Zhen Hu
    • Yang Huang
    • Xiao-Ming Wang
    • Xiu-Min Zhang
    • Yu-Jing Sun
    • Zeng-Shan Li
    • Shao-Yan Si
    • Yan-Fang Sui
  • View Affiliations

  • Published online on: October 1, 2009     https://doi.org/10.3892/or_00000517
  • Pages: 915-920
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Abstract

Our previous study showed that nanoemulsion-encapsulated MAGE1-HSP70/SEA (MHS) complex protein vaccine elicited MAGE-1 specific immune response and antitumor effects against MAGE-1-expressing tumor and nanoemulsion is a useful vehicle with possible important implications for cancer biotherapy. The purpose of this study was to compare the immune responses induced by nanoemulsion-encapsulated MAGE1-HSP70 and SEA as NE(MHS) vaccine following different administration routes and to find out the new and effective immune routes. Nanoemulsion vaccine was prepared using magnetic ultrasound methods. C57BL/6 mice were immunized with NE(MHS) via po., i.v., s.c. or i.p., besides mice s.c. injected with PBS or NE(−) as control. The cellular immunocompetence was detected by ELISpot assay and LDH release assay. The therapeutic and tumor challenge assay were also examined. The results showed that the immune responses against MAGE-1 expressing murine tumors elicited by NE(MHS) via 4 different routes were approximately similar and were all stronger than that elicited by PBS or NE(−), suggesting that this novel nanoemulsion carrier can exert potent antitumor immunity against antigens encapsulated in it. Especially, the present results indicated that nanoemulsion vaccine adapted to administration via different routes including peroral, and may have broader applications in the future.

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October 2009
Volume 22 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ge W, Hu P, Huang Y, Wang X, Zhang X, Sun Y, Li Z, Si S and Sui Y: The antitumor immune responses induced by nanoemulsion-encapsulated MAGE1-HSP70/SEA complex protein vaccine following different administration routes. Oncol Rep 22: 915-920, 2009
APA
Ge, W., Hu, P., Huang, Y., Wang, X., Zhang, X., Sun, Y. ... Sui, Y. (2009). The antitumor immune responses induced by nanoemulsion-encapsulated MAGE1-HSP70/SEA complex protein vaccine following different administration routes. Oncology Reports, 22, 915-920. https://doi.org/10.3892/or_00000517
MLA
Ge, W., Hu, P., Huang, Y., Wang, X., Zhang, X., Sun, Y., Li, Z., Si, S., Sui, Y."The antitumor immune responses induced by nanoemulsion-encapsulated MAGE1-HSP70/SEA complex protein vaccine following different administration routes". Oncology Reports 22.4 (2009): 915-920.
Chicago
Ge, W., Hu, P., Huang, Y., Wang, X., Zhang, X., Sun, Y., Li, Z., Si, S., Sui, Y."The antitumor immune responses induced by nanoemulsion-encapsulated MAGE1-HSP70/SEA complex protein vaccine following different administration routes". Oncology Reports 22, no. 4 (2009): 915-920. https://doi.org/10.3892/or_00000517