A role of functional T-type Ca2+ channel in hepatocellular carcinoma cell proliferation

  • Authors:
    • Yajuan Li
    • Shuang Liu
    • Fengmin Lu
    • Ting Zhang
    • Hairu Chen
    • Songwei Wu
    • Hui Zhuang
  • View Affiliations

  • Published online on: November 1, 2009     https://doi.org/10.3892/or_00000559
  • Pages: 1229-1235
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Abstract

The role of T-type Ca2+ channels in hepatocellular carcinoma cell proliferation was investigated in vitro. Eleven hepatocellular carcinoma cell lines and one immortalized liver cell line (LO2) were examined for the status of T-type Ca2+ channels with RT-PCR and voltage-clamp recordings. Except HBxF344, other cell lines tested had one, two or all three of α1-subunits (α1G, α1H and α1I) mRNA expression. Obvious T-type current was recorded in SNU449 cells, while others exhibited a minimal or no T-type current. SNU449, PLC/PRF5, Hep3B and LO2 cell lines were subjected to growth assay in the presence of Mibefradil, a T-type Ca2+ channel blocker, only the proliferation of SNU449 cell which had functional T-type Ca2+ channel was reduced by Mibefradil treatment. Furthermore, the persistent increase of phosphorylated ERK1/2 in SNU449 cells was found when treated with Mibefradil. A microarray assay also demonstrated some down-regulated genes were mainly associated with cell cycle and cell proliferation in Mibefradil treated SNU449 cells. In conclusion, this study showed that the functional T-type Ca2+ channels probably participate in modulating the proliferation of some hepatocellular carcinoma cells. The cell proliferation reduction of SNU449 with Mibefradil treatment is possibly associated with the persistent increase of phosphorylated ERK1/2.

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November 2009
Volume 22 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Li Y, Liu S, Lu F, Zhang T, Chen H, Wu S and Zhuang H: A role of functional T-type Ca2+ channel in hepatocellular carcinoma cell proliferation. Oncol Rep 22: 1229-1235, 2009
APA
Li, Y., Liu, S., Lu, F., Zhang, T., Chen, H., Wu, S., & Zhuang, H. (2009). A role of functional T-type Ca2+ channel in hepatocellular carcinoma cell proliferation. Oncology Reports, 22, 1229-1235. https://doi.org/10.3892/or_00000559
MLA
Li, Y., Liu, S., Lu, F., Zhang, T., Chen, H., Wu, S., Zhuang, H."A role of functional T-type Ca2+ channel in hepatocellular carcinoma cell proliferation". Oncology Reports 22.5 (2009): 1229-1235.
Chicago
Li, Y., Liu, S., Lu, F., Zhang, T., Chen, H., Wu, S., Zhuang, H."A role of functional T-type Ca2+ channel in hepatocellular carcinoma cell proliferation". Oncology Reports 22, no. 5 (2009): 1229-1235. https://doi.org/10.3892/or_00000559