The ubiquitin-like molecule interferon-stimulated gene 15 is overexpressed in human prostate cancer

  • Authors:
    • Hirofumi Satake
    • Kenji Tamura
    • Mutsuo Furihata
    • Takashi Anchi
    • Hatsune Sakoda
    • Chiaki Kawada
    • Tatsuo Iiyama
    • Shingo Ashida
    • Taro Shuin
  • View Affiliations

  • Published online on: January 1, 2010     https://doi.org/10.3892/or_00000600
  • Pages: 11-16
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Abstract

To identify molecules to serve as diagnostic markers for high-grade prostate cancer (PC) and targets for novel therapeutic drugs, we investigated the gene expression profiles of high-grade PCs using a cDNA microarray combined with laser microbeam microdissection. We subsequently confirmed that the ubiquitin-like molecule interferon-stimulated gene 15 (ISG15) was expressed exclusively in high-grade PCs with high Gleason scores. Semi-quantitative reverse transcription PCR and immunohistochemical analysis confirmed the overexpression of ISG15, a 165 amino acid interferon-inducible ubiquitin-like protein, specifically in high-grade PCs with high Gleason scores 8-9, while it was not expressed in the normal prostate. Immunohistochemical analysis using anti-ISG15 polyclonal antibody confirmed an elevated expression of ISG15 protein in high-grade PCs as well as low-grade PCs compared with that in normal prostate (NP) epithelium. Knockdown of ISG15 expression by short interfering RNA (siRNA) in a PC cell line resulted in marked attenuation of PC cell survival; concordantly, ISG15 overexpression in a PC cell line promoted PC cell growth, indicating its oncogenic property. These findings suggest that ISG15 is involved in cell growth and survival of PCs and that it could be a potential molecular target for new therapeutics and a diagnostic biomarker for human PCs.

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January 2010
Volume 23 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Satake H, Tamura K, Furihata M, Anchi T, Sakoda H, Kawada C, Iiyama T, Ashida S and Shuin T: The ubiquitin-like molecule interferon-stimulated gene 15 is overexpressed in human prostate cancer. Oncol Rep 23: 11-16, 2010
APA
Satake, H., Tamura, K., Furihata, M., Anchi, T., Sakoda, H., Kawada, C. ... Shuin, T. (2010). The ubiquitin-like molecule interferon-stimulated gene 15 is overexpressed in human prostate cancer. Oncology Reports, 23, 11-16. https://doi.org/10.3892/or_00000600
MLA
Satake, H., Tamura, K., Furihata, M., Anchi, T., Sakoda, H., Kawada, C., Iiyama, T., Ashida, S., Shuin, T."The ubiquitin-like molecule interferon-stimulated gene 15 is overexpressed in human prostate cancer". Oncology Reports 23.1 (2010): 11-16.
Chicago
Satake, H., Tamura, K., Furihata, M., Anchi, T., Sakoda, H., Kawada, C., Iiyama, T., Ashida, S., Shuin, T."The ubiquitin-like molecule interferon-stimulated gene 15 is overexpressed in human prostate cancer". Oncology Reports 23, no. 1 (2010): 11-16. https://doi.org/10.3892/or_00000600