Propyl gallate inhibits the growth of HeLa cells via caspase-dependent apoptosis as well as a G1 phase arrest of the cell cycle

  • Authors:
    • Yong Hwan Han
    • Hwa Jin Moon
    • Bo Ra You
    • Sung Zoo Kim
    • Suhn Hee Kim
    • Woo Hyun Park
  • View Affiliations

  • Published online on: April 1, 2010     https://doi.org/10.3892/or_00000745
  • Pages: 1153-1158
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Abstract

Propyl gallate (PG) as a synthetic antioxidant exerts a variety of effects on tissue and cell functions. Here, we evaluated the effects of PG on the growth of HeLa cells in relation to apoptosis and the cell cycle. PG dose-dependently inhibited the growth of HeLa cells with an IC50 of approximately 800 µM at 24 h. DNA flow cytometric analysis indicated that PG significantly induced a G1 phase arrest of the cell cycle along with an increase in the cyclin-dependent kinase inhibitor (CDKI) p27. In addition, PG induced apoptosis, which was accompanied by the loss of mitochondrial membrane potential (MMP; ΔΨm), activation of caspase-3 and caspase-8 and PARP cleavage. All the tested caspase inhibitors (pan-caspase, caspase-3, -8 or -9 inhibitor) significantly rescued HeLa cells from PG-induced cell death. However, none of the caspase inhibitors prevented the loss of MMP (ΔΨm) induced by PG. In conclusion, PG inhibited the growth of HeLa cells via caspase-dependent apoptosis as well as a G1 phase arrest of the cell cycle.

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April 2010
Volume 23 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Han YH, Moon HJ, You BR, Kim SZ, Kim SH and Park WH: Propyl gallate inhibits the growth of HeLa cells via caspase-dependent apoptosis as well as a G1 phase arrest of the cell cycle. Oncol Rep 23: 1153-1158, 2010
APA
Han, Y.H., Moon, H.J., You, B.R., Kim, S.Z., Kim, S.H., & Park, W.H. (2010). Propyl gallate inhibits the growth of HeLa cells via caspase-dependent apoptosis as well as a G1 phase arrest of the cell cycle. Oncology Reports, 23, 1153-1158. https://doi.org/10.3892/or_00000745
MLA
Han, Y. H., Moon, H. J., You, B. R., Kim, S. Z., Kim, S. H., Park, W. H."Propyl gallate inhibits the growth of HeLa cells via caspase-dependent apoptosis as well as a G1 phase arrest of the cell cycle". Oncology Reports 23.4 (2010): 1153-1158.
Chicago
Han, Y. H., Moon, H. J., You, B. R., Kim, S. Z., Kim, S. H., Park, W. H."Propyl gallate inhibits the growth of HeLa cells via caspase-dependent apoptosis as well as a G1 phase arrest of the cell cycle". Oncology Reports 23, no. 4 (2010): 1153-1158. https://doi.org/10.3892/or_00000745