AF1q enhancement of γ irradiation-induced apoptosis by up-regulation of BAD expression via NF-κB in human squamous carcinoma A431 cells

  • Authors:
    • Ngai Na Co
    • Wing Pui Tsang
    • Tsun Yee Tsang
    • Chi Lam Au Yeung
    • Pak Lun Yau
    • Siu Kai Kong
    • Tim Tak Kwok
  • View Affiliations

  • Published online on: August 1, 2010     https://doi.org/10.3892/or_00000891
  • Pages: 547-554
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Abstract

BAD (BCL-2 antagonist of cell death) is a pro-apoptotic BCL-2 family protein that plays a critical role in the regulation of apoptotic response. This study presents direct evidence that AF1q increased the radiation-induced apoptosis through up-regulation of BAD in human squamous carcinoma A431 cells and the key transcription factor involved is NF-κB. The minimal promoter sequence of BAD was identified; the activity was increased in AF1q stable transfectants and decreased upon AF1q siRNA transfection. The NF-κB consensus binding sequence is detected on BAD promoter. Inactivation of NF-κB by NF-κB inhibitor Bay 11-7082 or NF-κB p65 siRNA suppressed the expression and promoter activity of BAD; the suppression is more obvious in AF1q stable transfectants which also have an elevated NF-κB level. Mutation of putative NF-κB motif decreased the BAD promoter activity. The binding of NF-κB to the BAD promoter was confirmed by chromatin-immunoprecipitation. These findings indicate that AF1q up-regulation of BAD is through its effect on NF-κB and this may hint of its oncogenic mechanism in cancer.

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August 2010
Volume 24 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Co NN, Tsang WP, Tsang TY, Yeung CL, Yau PL, Kong SK and Kwok TT: AF1q enhancement of γ irradiation-induced apoptosis by up-regulation of BAD expression via NF-κB in human squamous carcinoma A431 cells. Oncol Rep 24: 547-554, 2010.
APA
Co, N.N., Tsang, W.P., Tsang, T.Y., Yeung, C.L., Yau, P.L., Kong, S.K., & Kwok, T.T. (2010). AF1q enhancement of γ irradiation-induced apoptosis by up-regulation of BAD expression via NF-κB in human squamous carcinoma A431 cells. Oncology Reports, 24, 547-554. https://doi.org/10.3892/or_00000891
MLA
Co, N. N., Tsang, W. P., Tsang, T. Y., Yeung, C. L., Yau, P. L., Kong, S. K., Kwok, T. T."AF1q enhancement of γ irradiation-induced apoptosis by up-regulation of BAD expression via NF-κB in human squamous carcinoma A431 cells". Oncology Reports 24.2 (2010): 547-554.
Chicago
Co, N. N., Tsang, W. P., Tsang, T. Y., Yeung, C. L., Yau, P. L., Kong, S. K., Kwok, T. T."AF1q enhancement of γ irradiation-induced apoptosis by up-regulation of BAD expression via NF-κB in human squamous carcinoma A431 cells". Oncology Reports 24, no. 2 (2010): 547-554. https://doi.org/10.3892/or_00000891