High-level transgene expression mediated by the piggyBac transposon enhances transgenic therapeutic effects in cervical cancer xenografts

  • Authors:
    • Yu Kang
    • Wenbo Yu
    • Qinwen Sun
    • Xiaoyan Zhang
    • Wei Jiang
    • Chaoqun Wu
    • Chunmei Chen
    • Jianren Gu
    • Yufang Zheng
    • Congjian Xu
  • View Affiliations

  • Published online on: October 1, 2010     https://doi.org/10.3892/or_00000935
  • Pages: 897-907
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Abstract

The piggyBac (PB) transposon is a recently identified, active and flexible transgene vector, combining the advantages of non-viral gene delivery with genomic integration and persistent transgene expression. In this study, we utilized the PB transposon to carry the herpes simplex thymidine kinase (HSV-tk) and red fluorescent protein (mRFP1) reporter genes into the HeLa cervical cancer cell line or tumor xenografts of cervical cancer. Our data showed that HSV-tk and mRFP1 were expressed in HeLa cells and tumor xenografts three weeks after intratumoral injection. The mRNA and protein levels of HSV-tk and mRFP1 were increased by using the PB transposon vector. Our system also demonstrated that sensitivity of transfected HeLa cells to the pro-drug ganciclovir (GCV) was enhanced in vitro and in vivo. Furthermore, our data indicated that the enhanced transgenic therapeutic effect was strongly associated with high-level transgene expression mediated by the PB transposon. Our results suggest that applying the PB transposon in HSV-tk gene delivery and GCV treatment is a promising gene therapy strategy in the treatment of cervical cancer.

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October 2010
Volume 24 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kang Y, Yu W, Sun Q, Zhang X, Jiang W, Wu C, Chen C, Gu J, Zheng Y, Xu C, Xu C, et al: High-level transgene expression mediated by the piggyBac transposon enhances transgenic therapeutic effects in cervical cancer xenografts . Oncol Rep 24: 897-907, 2010.
APA
Kang, Y., Yu, W., Sun, Q., Zhang, X., Jiang, W., Wu, C. ... Xu, C. (2010). High-level transgene expression mediated by the piggyBac transposon enhances transgenic therapeutic effects in cervical cancer xenografts . Oncology Reports, 24, 897-907. https://doi.org/10.3892/or_00000935
MLA
Kang, Y., Yu, W., Sun, Q., Zhang, X., Jiang, W., Wu, C., Chen, C., Gu, J., Zheng, Y., Xu, C."High-level transgene expression mediated by the piggyBac transposon enhances transgenic therapeutic effects in cervical cancer xenografts ". Oncology Reports 24.4 (2010): 897-907.
Chicago
Kang, Y., Yu, W., Sun, Q., Zhang, X., Jiang, W., Wu, C., Chen, C., Gu, J., Zheng, Y., Xu, C."High-level transgene expression mediated by the piggyBac transposon enhances transgenic therapeutic effects in cervical cancer xenografts ". Oncology Reports 24, no. 4 (2010): 897-907. https://doi.org/10.3892/or_00000935