Open Access

Characterization of an alternative transcript of the human CREB3L2 gene

  • Authors:
    • Ioannis Panagopoulos
    • Nastaran Monsef
    • Anna Collin
    • Fredrik Mertens
  • View Affiliations

  • Published online on: November 1, 2010     https://doi.org/10.3892/or_00000964
  • Pages: 1133-1139
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Abstract

CREB3L2, a member of the CREB3 family of transcription factors, spans >120 kbp and is composed of 12 exons. We characterized a widely expressed transcript of CREB3L2 generated by an intronic polyadenylation site in intron 4 of the gene. It could be translated to a CREB3L2 variant which is localized both in the nucleus and the endoplasmatic reticulum. The protein retains the N-terminal transactivation domain but lacks the DNA-binding domain, the transmembrane domain and the C-terminal part. Experiments using a GAL4 DNA-binding domain fusion model showed that the transcript is a transactivator but it cannot exert its function through the CRE and ATF6 binding sites and has little effect on the GRP78 promoter. Whether this transcript has a cellular function or is targeted for degradation by nonsense-mediated RNA decay system of RNA surveillance is currently unknown.

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November 2010
Volume 24 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Panagopoulos I, Monsef N, Collin A and Mertens F: Characterization of an alternative transcript of the human CREB3L2 gene . Oncol Rep 24: 1133-1139, 2010.
APA
Panagopoulos, I., Monsef, N., Collin, A., & Mertens, F. (2010). Characterization of an alternative transcript of the human CREB3L2 gene . Oncology Reports, 24, 1133-1139. https://doi.org/10.3892/or_00000964
MLA
Panagopoulos, I., Monsef, N., Collin, A., Mertens, F."Characterization of an alternative transcript of the human CREB3L2 gene ". Oncology Reports 24.5 (2010): 1133-1139.
Chicago
Panagopoulos, I., Monsef, N., Collin, A., Mertens, F."Characterization of an alternative transcript of the human CREB3L2 gene ". Oncology Reports 24, no. 5 (2010): 1133-1139. https://doi.org/10.3892/or_00000964