Cooperation of actin-sequestering protein, thymosin β-4 and hypoxia inducible factor-1α in tumor cell migration

  • Authors:
    • Yun-Kyoung Ryu
    • Yun-Sun Im
    • Eun-Yi Moon
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  • Published online on: November 1, 2010     https://doi.org/10.3892/or_00000997
  • Pages: 1389-1394
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Abstract

Cell migration plays an important role in many physiological and pathological processes, including tumor metastasis. Tumor cell migration is increased through the sequential induction of HIF-1α and VEGF under hypoxic conditions. Thymosin β-4 (Tβ4) is an actin-sequestering protein which controls cytoskeletal reorganization. Here, we investigated whether tumor cell migration could be co-operatively controlled by hypoxia and Tβ4. Cell migration was measured by wound healing assay with scratching confluent monolayers of tumor cells. Cell migration was enhanced 18 h after scratching cells. In addition, we found that the expression of HIF-1α, VEGF-A isoform 164/120 and Tβ4 was increased by scratching cells. Cell migration was decreased by the inhibition of Tβ4 or HIF-1α expression with lentiviral shRNA of Tβ4 or siRNA of HIF-1α, respectively. In contrast, cell migration was increased by the treatment with Tβ4 proteins. The inhibitory effect of Tβ4-shRNA or HIF-1α-siRNA was also attenuated by treatment with Tβ4 proteins. Collectively, these findings suggest that Tβ4 and HIF-1α cooperatively enhance tumor cell migration.

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November 2010
Volume 24 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ryu Y, Im Y and Moon E: Cooperation of actin-sequestering protein, thymosin β-4 and hypoxia inducible factor-1α in tumor cell migration . Oncol Rep 24: 1389-1394, 2010
APA
Ryu, Y., Im, Y., & Moon, E. (2010). Cooperation of actin-sequestering protein, thymosin β-4 and hypoxia inducible factor-1α in tumor cell migration . Oncology Reports, 24, 1389-1394. https://doi.org/10.3892/or_00000997
MLA
Ryu, Y., Im, Y., Moon, E."Cooperation of actin-sequestering protein, thymosin β-4 and hypoxia inducible factor-1α in tumor cell migration ". Oncology Reports 24.5 (2010): 1389-1394.
Chicago
Ryu, Y., Im, Y., Moon, E."Cooperation of actin-sequestering protein, thymosin β-4 and hypoxia inducible factor-1α in tumor cell migration ". Oncology Reports 24, no. 5 (2010): 1389-1394. https://doi.org/10.3892/or_00000997