Regulation of mutual inhibitory activities between AMPK and Akt with quercetin in MCF-7 breast cancer cells

  • Authors:
    • Yun-Kyoung Lee
    • Ock Jin Park
  • View Affiliations

  • Published online on: December 1, 2010     https://doi.org/10.3892/or_00001010
  • Pages: 1493-1497
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Abstract

In lieu of elucidating bidirectional connecting mechanism between AMP-activated protein kinase (AMPK) and survival signal Akt we applied MCF-7 breast cancer cells to determine whether AMPK modulation alters Akt signals and vice versa. Suppression of Akt activities with a synthetic Akt inhibitor alleviated AMPK activities suggesting that Akt is capable of inhibiting AMPK. Also the activation of AMPK with quercetin strongly abrogated Akt activities. Treating cancer cells with AMPK siRNA or Compound C resulted in marked increment of Akt dephosphorylation indicating that AMPK has antagonistic activities towards Akt. However, quercetin exerted Akt inhibitory activities in the absence of AMPK activation. Quercetin induced partial co-localization of phospho-Akt and phospho-AMPK in the nucleus even though their interaction seems to be indirect since the immunoprecipitation data indicate there was no direct binding between total Akt and AMPK. These results suggest there is a mutual suppressive interaction between AMPK and Akt. The investigation of mutual suppression between Akt and AMPK by chemo-preventive agents such as quercetin may provide a mechanistic rational for controlling breast tumor cell growth.

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December 2010
Volume 24 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Lee Y and Park OJ: Regulation of mutual inhibitory activities between AMPK and Akt with quercetin in MCF-7 breast cancer cells . Oncol Rep 24: 1493-1497, 2010.
APA
Lee, Y., & Park, O.J. (2010). Regulation of mutual inhibitory activities between AMPK and Akt with quercetin in MCF-7 breast cancer cells . Oncology Reports, 24, 1493-1497. https://doi.org/10.3892/or_00001010
MLA
Lee, Y., Park, O. J."Regulation of mutual inhibitory activities between AMPK and Akt with quercetin in MCF-7 breast cancer cells ". Oncology Reports 24.6 (2010): 1493-1497.
Chicago
Lee, Y., Park, O. J."Regulation of mutual inhibitory activities between AMPK and Akt with quercetin in MCF-7 breast cancer cells ". Oncology Reports 24, no. 6 (2010): 1493-1497. https://doi.org/10.3892/or_00001010