Terpenic subfraction of Pterodon pubescens induces apoptosis of K562 leukemic cells by modulating gene expression

  • Authors:
    • Monica Farah Pereira
    • Thiago Martino
    • Sergio Ranto Dalmau
    • Rodolfo Mattos Albano
    • Jean-Pierre Férézou
    • Sonia Soares Costa
    • Marsen Garcia Pinto Coelho
    • Kátia Gosta Carvalho Sabino
  • View Affiliations

  • Published online on: January 1, 2011     https://doi.org/10.3892/or_00001063
  • Pages: 215-221
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Abstract

Deregulation of cell proliferation and apoptosis is linked to malignant cell development. Leukemia is the most frequent cancer in children, and plants are important sources for new potential anti-cancer agents. Although anti-tumoral effects have been shown for Pterodon pubescens extracts, the mechanisms are still obscure. This study describes in Pterodon pubescens a furane diterpene only reported in Pterodon polygalaeflorus, the methyl-6α-acetoxy-7β-hydroxyvouacapan-17β-oate, indicated by HRMS and 13C-NMR analysis, and demonstrates some mechanisms of the anti-leukemia action of its terpene subfraction SF5. SF5 induced cytotoxic and anti-proliferative effects on K562 cells. Increased sub-G1 nuclei and Annexin V+-FITC cells confirmed apoptosis of leukemic cells by treatment of these cells with SF5. Down-regulation of DNMT1 gene transcription and over-expression of Apaf-1 mRNA suggested that SF5 may be inducing apoptosis of K562 cells by epigenetic up-regulation of pro-apoptotic proteins involved in the mitochondrial intrinsic pathway.

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January 2011
Volume 25 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Pereira MF, Martino T, Dalmau SR, Albano RM, Férézou J, Costa SS, Coelho MG and Sabino KG: Terpenic subfraction of Pterodon pubescens induces apoptosis of K562 leukemic cells by modulating gene expression . Oncol Rep 25: 215-221, 2011
APA
Pereira, M.F., Martino, T., Dalmau, S.R., Albano, R.M., Férézou, J., Costa, S.S. ... Sabino, K.G. (2011). Terpenic subfraction of Pterodon pubescens induces apoptosis of K562 leukemic cells by modulating gene expression . Oncology Reports, 25, 215-221. https://doi.org/10.3892/or_00001063
MLA
Pereira, M. F., Martino, T., Dalmau, S. R., Albano, R. M., Férézou, J., Costa, S. S., Coelho, M. G., Sabino, K. G."Terpenic subfraction of Pterodon pubescens induces apoptosis of K562 leukemic cells by modulating gene expression ". Oncology Reports 25.1 (2011): 215-221.
Chicago
Pereira, M. F., Martino, T., Dalmau, S. R., Albano, R. M., Férézou, J., Costa, S. S., Coelho, M. G., Sabino, K. G."Terpenic subfraction of Pterodon pubescens induces apoptosis of K562 leukemic cells by modulating gene expression ". Oncology Reports 25, no. 1 (2011): 215-221. https://doi.org/10.3892/or_00001063