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The mutant isocitrate dehydrogenase 1 product, 2‑hydroxyglutarate, activates MutT homolog 1 in glioma cells via the augmentation of reactive oxygen species levels

  • Authors:
    • Bharathan Bhavya
    • Chellapan Reghuvaran Anand
    • Madhusoodanan Urulangodi
    • Kalapurakkal Sreelakshmi
    • Akkihebbal Narasimhaiah Deepti
    • Girish Ramachandran Menon
    • Krishnakumar Kesavapisharady
    • Hariharan Venkat Easwer
    • Srinivas Gopala
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala 695011, India, Department of Pathology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala 695011, India, Department of Neurosurgery, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala 695011, India
    Copyright: © Bhavya et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 39
    |
    Published online on: October 3, 2022
       https://doi.org/10.3892/wasj.2022.174
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Abstract

MutT homolog1 (MTH1) is an enzyme responsible for removing oxidized nucleotides from cells. The activation of MTH1 has been reported in a number of cancer cell types and is considered to be responsible for imparting resistance towards anticancer drugs. While there are several known mechanisms for the activation of MTH1 in cancer cells, the present study aimed to evaluate the role of mutant isocitrate dehydrogenase1 (mIDH1)‑mediated reactive oxygen species (ROS) production in the activation of MTH1 in glioma cells. MTH1 was found to be upregulated in both mIDH1‑expressing cells and 2-hydroxyglutarate (2‑HG)‑treated cells. mIDH1 and its product, 2‑HG, increased the levels of ROS in cultured glioblastoma cells. Furthermore, the increased expression and activity of MTH1 were observed in glioma tissues harboring mIDH1 compared to tissues with wild‑type IDH1. On the whole, the findings of the present study unveil a novel mechanism of activation of MTH1 in glioma cells harboring mutant IDH1.
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Copy and paste a formatted citation
Spandidos Publications style
Bhavya B, Anand CR, Urulangodi M, Sreelakshmi K, Deepti AN, Menon GR, Kesavapisharady K, Easwer HV and Gopala S: The mutant isocitrate dehydrogenase 1 product, 2‑hydroxyglutarate, activates MutT homolog 1 in glioma cells via the augmentation of reactive oxygen species levels. World Acad Sci J 4: 39, 2022.
APA
Bhavya, B., Anand, C.R., Urulangodi, M., Sreelakshmi, K., Deepti, A.N., Menon, G.R. ... Gopala, S. (2022). The mutant isocitrate dehydrogenase 1 product, 2‑hydroxyglutarate, activates MutT homolog 1 in glioma cells via the augmentation of reactive oxygen species levels. World Academy of Sciences Journal, 4, 39. https://doi.org/10.3892/wasj.2022.174
MLA
Bhavya, B., Anand, C. R., Urulangodi, M., Sreelakshmi, K., Deepti, A. N., Menon, G. R., Kesavapisharady, K., Easwer, H. V., Gopala, S."The mutant isocitrate dehydrogenase 1 product, 2‑hydroxyglutarate, activates MutT homolog 1 in glioma cells via the augmentation of reactive oxygen species levels". World Academy of Sciences Journal 4.6 (2022): 39.
Chicago
Bhavya, B., Anand, C. R., Urulangodi, M., Sreelakshmi, K., Deepti, A. N., Menon, G. R., Kesavapisharady, K., Easwer, H. V., Gopala, S."The mutant isocitrate dehydrogenase 1 product, 2‑hydroxyglutarate, activates MutT homolog 1 in glioma cells via the augmentation of reactive oxygen species levels". World Academy of Sciences Journal 4, no. 6 (2022): 39. https://doi.org/10.3892/wasj.2022.174
Copy and paste a formatted citation
x
Spandidos Publications style
Bhavya B, Anand CR, Urulangodi M, Sreelakshmi K, Deepti AN, Menon GR, Kesavapisharady K, Easwer HV and Gopala S: The mutant isocitrate dehydrogenase 1 product, 2‑hydroxyglutarate, activates MutT homolog 1 in glioma cells via the augmentation of reactive oxygen species levels. World Acad Sci J 4: 39, 2022.
APA
Bhavya, B., Anand, C.R., Urulangodi, M., Sreelakshmi, K., Deepti, A.N., Menon, G.R. ... Gopala, S. (2022). The mutant isocitrate dehydrogenase 1 product, 2‑hydroxyglutarate, activates MutT homolog 1 in glioma cells via the augmentation of reactive oxygen species levels. World Academy of Sciences Journal, 4, 39. https://doi.org/10.3892/wasj.2022.174
MLA
Bhavya, B., Anand, C. R., Urulangodi, M., Sreelakshmi, K., Deepti, A. N., Menon, G. R., Kesavapisharady, K., Easwer, H. V., Gopala, S."The mutant isocitrate dehydrogenase 1 product, 2‑hydroxyglutarate, activates MutT homolog 1 in glioma cells via the augmentation of reactive oxygen species levels". World Academy of Sciences Journal 4.6 (2022): 39.
Chicago
Bhavya, B., Anand, C. R., Urulangodi, M., Sreelakshmi, K., Deepti, A. N., Menon, G. R., Kesavapisharady, K., Easwer, H. V., Gopala, S."The mutant isocitrate dehydrogenase 1 product, 2‑hydroxyglutarate, activates MutT homolog 1 in glioma cells via the augmentation of reactive oxygen species levels". World Academy of Sciences Journal 4, no. 6 (2022): 39. https://doi.org/10.3892/wasj.2022.174
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