Comparative study of biological characteristics of mesenchymal stem cells isolated from mouse bone marrow and peripheral blood

Lotfy,Ahmed; El‑Sherbiny,Yasser  M.; Cuthbert,Richard; Jones,Elena; Badawy,Ahmed

doi:10.3892/br.2019.1236

Comparative study of biological characteristics of mesenchymal stem cells isolated from mouse bone marrow and peripheral blood

Authors:
- Ahmed Lotfy
- Yasser M. El‑Sherbiny
- Richard Cuthbert
- Elena Jones
- Ahmed Badawy
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Affiliations: Biotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni‑Suef University, Beni‑Suef 62511, Egypt, Leeds Institute of Rheumatic and Musculoskeletal Medicine, University of Leeds, Leeds LS9 7TF, UK, Medical Experimental Research Center, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt
Published online on: September 2, 2019 https://doi.org/10.3892/br.2019.1236
Pages: 165-170

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Abstract

Mesenchymal stromal cells (MSCs) possess self‑renewal and multilineage differentiation potential, indicating their prospects as cellular therapeutic agents for regenerative medicine. Although adult bone marrow (BM) is the major source of these cells for clinical use, harvesting requires invasive procedures. Therefore, alternative sources, such as peripheral blood (PB), are needed. The objective of the current study was to compare PB‑MSCs and BM‑MSCs with regard to their biological characteristics. PB‑MSCs and BM‑MSCs were isolated from 4‑week‑old BALB/c white mice by density gradient centrifugation and cultured in DMEM + 10% fetal bovine serum until passage four. Morphological features, proliferation, cell surface marker expression and trilineage differentiation potential were assessed for both PB‑MSCs and BM‑MSCs. No significant differences in morphological features were observed. BM‑MSCs had a higher proliferative capability than PB‑MSCs as measured by XTT assays. Both PB‑MSCs and BM‑MSCs had broadly similar cell surface marker expression, but PB‑MSCs had positive expression of cluster of differentiation (CD)146 and CD140b. Both PB‑MSCs and BM‑MSCs were capable of trilineage differentiation. Although BM‑MSCs had a greater capacity for osteogenic and chondrogenic differentiation than PB‑MSCs, PB‑MSCs had a better capability for adipogenic differentiation than BM‑MSCs. In conclusion, PB‑MSCs and BM‑MSCs have very similar biological characteristics. Thus, PB is a promising source for easily obtaining MSCs in mice.

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1	Si YL, Zhao YL, Hao HJ, Fu XB and Han WD: MSCs: Biological characteristics, clinical applications and their outstanding concerns. Ageing Res Rev. 10:93–103. 2011.PubMed/NCBI View Article : Google Scholar
2	Vater C, Kasten P and Stiehler M: Culture media for the differentiation of mesenchymal stromal cells. Acta Biomater. 7:463–477. 2011.PubMed/NCBI View Article : Google Scholar
3	Yagi H, Soto-Gutierrez A, Parekkadan B, Kitagawa Y, Tompkins RG, Kobayashi N and Yarmush ML: Mesenchymal stem cells: Mechanisms of immunomodulation and homing. Cell Transplant. 19:667–679. 2010.PubMed/NCBI View Article : Google Scholar
4	Sohni A and Verfaillie CM: Mesenchymal stem cells migration homing and tracking. Stem Cells Int. 2013(130763)2013.PubMed/NCBI View Article : Google Scholar
5	Bianchi F, Sala E, Donadei C, Capelli I and La Manna G: Potential advantages of acute kidney injury management by mesenchymal stem cells. World J Stem Cells. 6:644–650. 2014.PubMed/NCBI View Article : Google Scholar
6	Friedenstein AJ, Chailakhyan RK, Latsinik NV, Panasyuk AF and Keiliss-Borok IV: Stromal cells responsible for transferring the microenvironment of the hemopoietic tissues. Cloning in vitro and retransplantation in vivo. Transplantation. 17:331–340. 1974.PubMed/NCBI View Article : Google Scholar
7	Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, Alfonso ZC, Fraser JK, Benhaim P and Hedrick MH: Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 13:4279–4295. 2002.PubMed/NCBI View Article : Google Scholar
8	Wang HS, Hung SC, Peng ST, Huang CC, Wei HM, Guo YJ, Fu YS, Lai MC and Chen CC: Mesenchymal stem cells in the Wharton's jelly of the human umbilical cord. Stem Cells. 22:1330–1337. 2004.PubMed/NCBI View Article : Google Scholar
9	Liu J, Yu F, Sun Y, Jiang B, Zhang W, Yang J, Xu GT, Liang A and Liu S: Concise reviews: Characteristics and potential applications of human dental tissue-derived mesenchymal stem cells. Stem Cells. 33:627–638. 2015.PubMed/NCBI View Article : Google Scholar
10	Friedenstein AJ, Chailakhjan RK and Lalykina KS: The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet. 3:393–403. 1970.PubMed/NCBI
11	Harasymiak-Krzyzanowska I, Niedojadlo A, Karwat J, Kotula L, Gil-Kulik P, Sawiuk M and Kocki J: Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications. Cell Mol Biol Lett. 18:479–493. 2013.PubMed/NCBI View Article : Google Scholar
12	Lotfy A, Salama M, Zahran F, Jones E, Badawy A and Sobh M: Characterization of mesenchymal stem cells derived from rat bone marrow and adipose tissue: A comparative study. Int J Stem Cells. 7:135–142. 2014.PubMed/NCBI View Article : Google Scholar
13	He Q, Wan C and Li G: Concise review: Multipotent mesenchymal stromal cells in blood. Stem Cells. 25:69–77. 2007.PubMed/NCBI View Article : Google Scholar
14	Koerner J, Nesic D, Romero JD, Brehm W, Mainil-Varlet P and Grogan SP: Equine peripheral blood-derived progenitors in comparison to bone marrow-derived mesenchymal stem cells. Stem Cells. 24:1613–1619. 2006.PubMed/NCBI View Article : Google Scholar
15	Zvaifler NJ, Marinova-Mutafchieva L, Adams G, Edwards CJ, Moss J, Burger JA and Maini RN: Mesenchymal precursor cells in the blood of normal individuals. Arthritis Res. 2:477–488. 2000.PubMed/NCBI View Article : Google Scholar
16	Fu WL, Zhang JY, Fu X, Duan XN, Leung KK, Jia ZQ, Wang WP, Zhou CY and Yu JK: Comparative study of the biological characteristics of mesenchymal stem cells from bone marrow and peripheral blood of rats. Tissue Eng Part A. 18:1793–1803. 2012.PubMed/NCBI View Article : Google Scholar
17	Longhini ALF, Salazar TE, Vieira C, Trinh T, Duan Y, Pay LM, Li Calzi S, Losh M, Johnston NA, Xie H, et al: Peripheral blood-derived mesenchymal stem cells demonstrate immunomodulatory potential for therapeutic use in horses. PLoS One. 14(e0212642)2019.PubMed/NCBI View Article : Google Scholar
18	Li S, Huang KJ, Wu JC, Hu MS, Sanyal M, Hu M, Longaker MT and Lorenz HP: Peripheral blood-derived mesenchymal stem cells: Candidate cells responsible for healing critical-sized calvarial bone defects. Stem Cells Transl Med. 4:359–368. 2015.PubMed/NCBI View Article : Google Scholar
19	Fu Q, Tang NN, Zhang Q, Liu Y, Peng JC, Fang N, Yu LM, Liu JW and Zhang T: Preclinical study of cell therapy for osteonecrosis of the femoral head with allogenic peripheral blood-derived mesenchymal stem cells. Yonsei Med J. 57:1006–1015. 2016.PubMed/NCBI View Article : Google Scholar
20	Fruehauf S, Veldwijk MR, Seeger T, Schubert M, Laufs S, Topaly J, Wuchter P, Dillmann F, Eckstein V, Wenz F, et al: A combination of granulocyte-colony-stimulating factor (G-CSF) and plerixafor mobilizes more primitive peripheral blood progenitor cells than G-CSF alone: Results of a European phase II study. Cytotherapy. 11:992–1001. 2009.PubMed/NCBI View Article : Google Scholar
21	Nadri S and Soleimani M: Isolation murine mesenchymal stem cells by positive selection. In Vitro Cell Dev Biol Anim. 43:276–282. 2007.PubMed/NCBI View Article : Google Scholar
22	Kuznetsov SA, Mankani MH, Gronthos S, Satomura K, Bianco P and Robey PG: Circulating skeletal stem cells. J Cell Biol. 153:1133–1140. 2001.PubMed/NCBI View Article : Google Scholar
23	Jones EA, English A, Henshaw K, Kinsey SE, Markham AF, Emery P and McGonagle D: Enumeration and phenotypic characterization of synovial fluid multipotential mesenchymal progenitor cells in inflammatory and degenerative arthritis. Arthritis Rheum. 50:817–827. 2004.PubMed/NCBI View Article : Google Scholar
24	Aldridge A, Kouroupis D, Churchman S, English A, Ingham E and Jones E: Assay validation for the assessment of adipogenesis of multipotential stromal cells-a direct comparison of four different methods. Cytotherapy. 15:89–101. 2013.PubMed/NCBI View Article : Google Scholar
25	Espagnolle N, Guilloton F, Deschaseaux F, Gadelorge M, Sensébé L and Bourin P: CD146 expression on mesenchymal stem cells is associated with their vascular smooth muscle commitment. J Cell Mol Med. 18:104–114. 2014.PubMed/NCBI View Article : Google Scholar
26	Lyahyai J, Mediano DR, Ranera B, Sanz A, Remacha AR, Bolea R, Zaragoza P, Rodellar C and Martín-Burriel I: Isolation and characterization of ovine mesenchymal stem cells derived from peripheral blood. BMC Vet Res. 8(169)2012.PubMed/NCBI View Article : Google Scholar
27	Spaas JH, De Schauwer C, Cornillie P, Meyer E, Van Soom A and Van de Walle GR: Culture and characterisation of equine peripheral blood mesenchymal stromal cells. Vet J. 195:107–113. 2013.PubMed/NCBI View Article : Google Scholar
28	Chong PP, Selvaratnam L, Abbas AA and Kamarul T: Human peripheral blood derived mesenchymal stem cells demonstrate similar characteristics and chondrogenic differentiation potential to bone marrow derived mesenchymal stem cells. J Orthop Res. 30:634–642. 2012.PubMed/NCBI View Article : Google Scholar
29	Hass R, Kasper C, Böhm S and Jacobs R: Different populations and sources of human mesenchymal stem cells (MSC): A comparison of adult and neonatal tissue-derived MSC. Cell Commun Signal. 9(12)2011.PubMed/NCBI View Article : Google Scholar