Recombinant expression and biological characterization of the antimicrobial peptide fowlicidin‑2 in Pichia pastoris

  • Authors:
    • Li‑Wei Xing
    • Shi‑Xun Tian
    • Wei Gao
    • Na Yang
    • Pei Qu
    • Di Liu
    • Jian Jiao
    • Jue Wang
    • Xing‑Jun Feng
  • View Affiliations

  • Published online on: August 5, 2016     https://doi.org/10.3892/etm.2016.3578
  • Pages: 2324-2330
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Abstract

Fowlicidins are a group of cathelicidin antimicrobial peptides that were initially identified in chickens. Fowlicidin-2, which is composed of 31 amino acids, is widely expressed in the majority of tissues in chickens and has an important role in innate immunity. In the present study, a recombinant expression system for fowlicidin‑2 was successfully constructed using Pichia pastoris X‑33 and the expression vector pPICZα‑A. Under the optimized fermentation conditions, 85.6 mg fowlicidin‑2 with >95% purity was obtained from 1 liter culture medium following purification by ion exchange chromatography and reversed phase high performance liquid chromatography. The recombinant fowlicidin‑2 exhibited broad spectrum antimicrobial activity and had a minimum inhibitory concentration ranging from 1 to 4 µM. Furthermore, recombinant fowlicidin‑2 exhibited hemolytic activity, promoting 50% human erythrocyte hemolysis in the concentration range of 128‑256 µM, and anticancer activity, resulting in the death of 50% of A375 human malignant melanoma cells in the concentration range of 2‑4 µM. The results of the present study suggest that recombinant fowlicidin‑2 may be a promising candidate for therapeutic applications.
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October-2016
Volume 12 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Xing LW, Tian SX, Gao W, Yang N, Qu P, Liu D, Jiao J, Wang J and Feng XJ: Recombinant expression and biological characterization of the antimicrobial peptide fowlicidin‑2 in Pichia pastoris. Exp Ther Med 12: 2324-2330, 2016
APA
Xing, L., Tian, S., Gao, W., Yang, N., Qu, P., Liu, D. ... Feng, X. (2016). Recombinant expression and biological characterization of the antimicrobial peptide fowlicidin‑2 in Pichia pastoris. Experimental and Therapeutic Medicine, 12, 2324-2330. https://doi.org/10.3892/etm.2016.3578
MLA
Xing, L., Tian, S., Gao, W., Yang, N., Qu, P., Liu, D., Jiao, J., Wang, J., Feng, X."Recombinant expression and biological characterization of the antimicrobial peptide fowlicidin‑2 in Pichia pastoris". Experimental and Therapeutic Medicine 12.4 (2016): 2324-2330.
Chicago
Xing, L., Tian, S., Gao, W., Yang, N., Qu, P., Liu, D., Jiao, J., Wang, J., Feng, X."Recombinant expression and biological characterization of the antimicrobial peptide fowlicidin‑2 in Pichia pastoris". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2324-2330. https://doi.org/10.3892/etm.2016.3578