Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors

Yu,Ziwei; Men,Yongzhi; Dong,Pin

doi:10.3892/etm.2017.4183

Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors

Authors:
- Ziwei Yu
- Yongzhi Men
- Pin Dong
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Affiliations: Department of Otolaryngology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, P.R. China
Published online on: March 6, 2017 https://doi.org/10.3892/etm.2017.4183
Pages: 2029-2035
Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study investigated whether co-culturing Schwann cells (SCs) with neural stem cells (NSCs) improves viability, direction of differentiation and secretion of brain‑derived neurotrophic factor (BDNF) and glial cell‑derived neurotrophic factor (GDNF) in NSCs. The three groups assessed were as follows: SCs, NSCs, and a co‑culture of SCs and NSCs. Cellular morphological changes were observed under an inverted phase contrast microscope and quantified. Cells were identified by immunofluorescence staining: S100 for SCs, Nestin for NSCs, microtubule associated protein (Map) 2 and NeuN for neurons and glial fibrillary acidic protein for astrocytes. Cell viability was evaluated by MTT assay. Secretion of BDNF and GDNF was quantified; mRNA expression was quantified by reverse transcription‑quantitative polymerase chain reaction. The majority of NSCs in the co‑cultured group differentiated into neurons. The cell survival rate of the co‑culture group was significantly higher than the other groups on days 3, 5 and 10 (P<0.01). The secretion of BDNF in the co‑culture group was significantly higher than NSCs on days 3, 5 and 7 (P<0.05), while the amount of GDNF in co‑culture was significantly higher than both NSCs and SCs on day 1 (P<0.05). BDNF and GDNF gene expression in the co‑culture group was significantly higher than SCs (P<0.01). Gene expression of Map2 in co‑culture group was also significantly higher than both NSC and SC groups (P<0.01). Therefore, co‑cultured SCs and NSCs promote differentiation of NSCs into neurons and secrete higher levels of neurotropic factors including BDNF and GDNF.

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