Open Access

Transcriptional profiling of leukocytes from rheumatoid arthritis patients before and after anti‑tumor necrosis factor therapy: A comparison of anti‑nuclear antibody positive and negative subsets

  • Authors:
    • Laurie S. Davis
    • Andreas M. Reimold
  • View Affiliations

  • Published online on: March 24, 2017     https://doi.org/10.3892/etm.2017.4265
  • Pages: 2183-2192
  • Copyright: © Davis et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Anti-nuclear antibodies (ANAs) may be induced in patients with rheumatoid arthritis (RA) receiving anti‑tumor necrosis factor (TNF) therapy with TNF inhibitors (TNFi), etanercept, infliximab or adalimumab. In the present study, 11 patients who were TNFi drug naive were started on TNFi at a time of high disease activity. Of these, all cases were positive for rheumatoid factor and 9 cases tested were positive for anti‑citrullinated peptide (anti‑CCP) antibodies prior to TNFi treatment. Peripheral blood mononuclear cells (PBMCs) and serum were collected from all patients before and after TNFi therapy. Serum was assayed for ANAs over time. Total cellular RNA was extracted from PBMCs and assessed using Illumina arrays. Gene expression profiles were examined for alterations in key effector pathways. After 3 or more months on TNFi, 6 patients converted to ANA‑positivity. Analysis of transcripts from patients with RA who converted to ANA‑positivity after 3 months on TNFi identified complex gene expression profiles that reflected a reduction in cell adhesion, cell stress and lipid metabolism transcripts. In summary, unique transcriptional profiles in PBMCs from patients with RA were observed after TNFi therapy. This pilot study suggests that transcriptional profiling is a precise method of measuring the impact of TNFi therapies and reveals novel pathways that likely influence the immune response.
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May-2017
Volume 13 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Davis LS and Davis LS: Transcriptional profiling of leukocytes from rheumatoid arthritis patients before and after anti‑tumor necrosis factor therapy: A comparison of anti‑nuclear antibody positive and negative subsets. Exp Ther Med 13: 2183-2192, 2017
APA
Davis, L.S., & Davis, L.S. (2017). Transcriptional profiling of leukocytes from rheumatoid arthritis patients before and after anti‑tumor necrosis factor therapy: A comparison of anti‑nuclear antibody positive and negative subsets. Experimental and Therapeutic Medicine, 13, 2183-2192. https://doi.org/10.3892/etm.2017.4265
MLA
Davis, L. S., Reimold, A. M."Transcriptional profiling of leukocytes from rheumatoid arthritis patients before and after anti‑tumor necrosis factor therapy: A comparison of anti‑nuclear antibody positive and negative subsets". Experimental and Therapeutic Medicine 13.5 (2017): 2183-2192.
Chicago
Davis, L. S., Reimold, A. M."Transcriptional profiling of leukocytes from rheumatoid arthritis patients before and after anti‑tumor necrosis factor therapy: A comparison of anti‑nuclear antibody positive and negative subsets". Experimental and Therapeutic Medicine 13, no. 5 (2017): 2183-2192. https://doi.org/10.3892/etm.2017.4265