Neuroprotective effects of phenylethanoid glycosides in an in vitro model of Alzheimer's disease

Yang,Jianhua; Ju,Bowei; Yan,Yao; Xu,Huanhuan; Wu,Shanshan; Zhu,Dandan; Cao,Dandan; Hu,Junping

doi:10.3892/etm.2017.4254

Neuroprotective effects of phenylethanoid glycosides in an in vitro model of Alzheimer's disease

Authors:
- Jianhua Yang
- Bowei Ju
- Yao Yan
- Huanhuan Xu
- Shanshan Wu
- Dandan Zhu
- Dandan Cao
- Junping Hu
View Affiliations

Affiliations: Department of Pharmacy, The First Affiliated Hospital, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China, Department of Natural Medicines, College of Pharmacy, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China
Published online on: March 22, 2017 https://doi.org/10.3892/etm.2017.4254
Pages: 2423-2428

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The present study aimed to investigate the neuroprotective effects of phenylethanol glycosides (PhGs) on H2O2‑ and β‑amyloid peptide (Aβ)1‑42‑induced injury of PC12 cells as an in vitro model of Alzheimer's disease (AD). The optimal induction conditions were established through screening of various incubation times and concentrations. PC12 cells were treated with 0.5 µM Aβ1‑42 and H2O2 in the presence of PhGs for 24 h and the cell viability was then evaluated by an MTT assay; lactate dehydrogenase (LDH) release and malondialdehyde (MDA) content were also measured. The optimal conditions for establishing the AD model were the treatment of PC12 cells with 0.5 µM Aβ1‑42 for 48 h, or with 25 µM H2O2 dissolved in DMEM with PBS. PhGs at concentrations of 5, 25 and 50 µg/ml increased the viability and decreased LDH and MDA release by PC12 cells injured with Aβ1‑42 or H2O2. In conclusion, the model of Aβ1‑42‑ and H2O2‑induced PC12 cell injury was successfully established. PhGs were shown to have a significant neuroprotective effect against Aβ1‑42‑ or H2O2‑induced cell injury.

View Figures

View References

1	Qu M, Zhou Z, Xu S, Chen C, Yu Z and Wang D: Mortalin overexpression attenuates beta-amyloid-induced neurotoxicity in SH-SY5Y cells. Brain Res. 1368:336–345. 2011. View Article : Google Scholar : PubMed/NCBI
2	Uzun S, Kozumplik O and Folnegović-Smalc V: Alzheimer's dementia: current data review. Collegium antropologicum. 35:1333–1337. 2011.PubMed/NCBI
3	Brookmeyer R, Johnson E, Ziegler-Graham K and Arrighi HM: Forecasting the global burden of Alzheimer's disease. Alzheimer's & dementia. 3:186–191. 2007. View Article : Google Scholar
4	Castellani RJ, Rolston RK and Smith MA: Alzheimer disease. Disease-a-month. 56:484–546. 2010. View Article : Google Scholar : PubMed/NCBI
5	Mattson MP: Pathways towards and away from Alzheimer's disease. Nature. 430:631–639. 2004. View Article : Google Scholar : PubMed/NCBI
6	Gouras GK, Tsai J, Naslund J, et al: Intraneuronal Aβ42 accumulation in human brain. The American journal of pathology. 156:15–20. 2000. View Article : Google Scholar : PubMed/NCBI
7	Shen C, Chen Y, Liu H, et al: Hydrogen peroxide promotes Aβ production through JNK-dependent activation of γ-secretase. Journal of Biological Chemistry. 283:17721–17730. 2008. View Article : Google Scholar : PubMed/NCBI
8	Shih P-H, Wu C-H, Yeh C-T and Yen G-C: Protective effects of anthocyanins against amyloid β-peptide-induced damage in neuro-2A cells. Journal of agricultural and food chemistry. 59:1683–1689. 2011. View Article : Google Scholar : PubMed/NCBI
9	Figueiredo CP, Bicca MA, Latini A, Prediger R, Medeiros R and Calixto JB: Folic acid plus α-tocopherol mitigates amyloid-β-induced neurotoxicity through modulation of mitochondrial complexes activity. Journal of Alzheimer's disease: JAD. 24:61–75. 2010.
10	Dumont M, Lin MT and Beal MF: Mitochondria and antioxidant targeted therapeutic strategies for Alzheimer's disease. Journal of Alzheimer's disease: JAD. 20:S6332010. View Article : Google Scholar
11	Sonnen JA, Breitner JC, Lovell MA, Markesbery WR, Quinn JF and Montine TJ: Free radical-mediated damage to brain in Alzheimer's disease and its transgenic mouse models. Free Radical Biology and Medicine. 45:219–230. 2008. View Article : Google Scholar : PubMed/NCBI
12	Lin MT and Beal MF: Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases. Nature. 443:787–795. 2006. View Article : Google Scholar : PubMed/NCBI
13	Trushina E and McMurray C: Oxidative stress and mitochondrial dysfunction in neurodegenerative diseases. Neuroscience. 145:1233–1248. 2007. View Article : Google Scholar : PubMed/NCBI
14	Huang S-H, Lin C-M and Chiang B-H: Protective effects of Angelica sinensis extract on amyloid β-peptide-induced neurotoxicity. Phytomedicine. 15:710–721. 2008. View Article : Google Scholar : PubMed/NCBI
15	Burhans WC and Heintz NH: The cell cycle is a redox cycle: linking phase-specific targets to cell fate. Free Radical Biology and Medicine. 47:1282–1293. 2009. View Article : Google Scholar : PubMed/NCBI
16	Xue HY, Gao GZ, Lin QY, Jin LJ and Xu YP: Protective effects of aucubin on H2O2-induced apoptosis in PC12 cells. Phytotherapy Research. 26:369–374. 2012.PubMed/NCBI
17	Li X, Gou C, Yang H, Qiu J, Gu T and Wen T: Echinacoside ameliorates D-galactosamine plus lipopolysaccharide-induced acute liver injury in mice via inhibition of apoptosis and inflammation. Scandinavian journal of gastroenterology. 49:993–1000. 2014. View Article : Google Scholar : PubMed/NCBI
18	Liu, F-X, Wang X-w, Luo L, Xin H, Na B and Wang X-F: The effects of glycosides of cistanche on learning and memory in beta-amyloid peptide induced Alzheimers disease in mice and its possible mechanism. Chinese Pharmacological Bulletin. 22:5952006.
19	Bao B, Tang X, Tian H, Tong Y, Wu W and Hong Y: Antioxidant activity of extracts from desert living Cistanche tubulosa (Schrenk) R. Wright Shanghai J Tradit Chin Med. 44:68–71. 2010.
20	Jiang Y, Li S, Wang Y, Chen X and Tu P: Differentiation of Herba Cistanches by fingerprint with high-performance liquid chromatography-diode array detection-mass spectrometry. Journal of Chromatography A. 1216:2156–2162. 2009. View Article : Google Scholar : PubMed/NCBI
21	Wang H, Xu Y, Yan J, et al: Acteoside protects human neuroblastoma SH-SY5Y cells against β-amyloid-induced cell injury. Brain research. 1283:139–147. 2009. View Article : Google Scholar : PubMed/NCBI
22	Zhao Q, Gao J, Li W and Cai D: Neurotrophic and neurorescue effects of Echinacoside in the subacute MPTP mouse model of Parkinson's disease. Brain research. 1346:224–236. 2010. View Article : Google Scholar : PubMed/NCBI
23	Kuang R, Sun Y, Yuan W, Lei L, Zheng X and Food Z: Protective effects of echinacoside, one of the phenylethanoid glycosides, on H2 O2-induced cytotoxicity in PC12 cells. neurodegenerative diseases. 8:92009.
24	Wu C-R, Lin H-C and Su M-H: Reversal by aqueous extracts of Cistanche tubulosa from behavioral deficits in Alzheimer's disease-like rat model: relevance for amyloid deposition and central neurotransmitter function. BMC complementary and alternative medicine. 14:2022014. View Article : Google Scholar : PubMed/NCBI
25	Cai RL, Yang MH, Shi Y, Chen J, Li YC and Qi Y: Antifatigue activity of phenylethanoid-rich extract from Cistanche deserticola. Phytotherapy research. 24:313–315. 2010. View Article : Google Scholar : PubMed/NCBI
26	Gao Y, Zong C, Liu F, et al: Evaluation of the Intestinal Transport of a Phenylethanoid Glycoside-Rich Extract from Cistanche deserticola across the Caco-2 Cell Monolayer Model. PloS one. 10:e01164902015. View Article : Google Scholar : PubMed/NCBI
27	Yoon J-H, Youn K, Ho C-T, Karwe MV, Jeong W-S and Jun M: p-Coumaric Acid and Ursolic Acid from Corni fructus Attenuated β-Amyloid 25–35-induced Toxicity through Regulation of the NF-κB Signaling Pathway in PC12 cells. Journal of agricultural and food chemistry. 62:4911–4916. 2014. View Article : Google Scholar : PubMed/NCBI
28	Zhu Y, Sun X, Gong T, He Q and Zhang Z: Antioxidant and Antiapoptotic Effects of 1, 1′-(Biphenyl-4,4′-diyl)-bis (3- (dimethylamino)-propan-1-one) on protecting PC12 cells from Aβ-induced injury. Molecular pharmaceutics. 11:428–435. 2013. View Article : Google Scholar : PubMed/NCBI
29	Chen Y, Su Y, Run X, et al: Pretreatment of PC12 cells with 17β-estradiol prevents Aβ-induced down-regulation of CREB phosphorylation and prolongs inhibition of GSK-3β. Journal of Molecular Neuroscience. 50:394–401. 2013. View Article : Google Scholar : PubMed/NCBI
30	Jiang B, Liu J, Bao Y and An L: Catalpol inhibits apoptosis in hydrogen peroxide-induced PC12 cells by preventing cytochrome c release and inactivating of caspase cascade. Toxicon. 43:53–59. 2004. View Article : Google Scholar : PubMed/NCBI