Open Access

Suppressive effect of microRNA-138 on the proliferation and invasion of osteosarcoma cells via targeting SIRT1

  • Authors:
    • Zhenchao Yuan
    • Hao Mo
    • Ligen Mo
    • Juliang He
    • Zhenjie Wu
    • Xiang Lin
  • View Affiliations

  • Published online on: May 4, 2017     https://doi.org/10.3892/etm.2017.4426
  • Pages: 3417-3423
  • Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRs), a class of small non-coding RNAs, function as key regulators in gene expression through binding to the 3'‑untranslated region (UTR) of their target mRNA, which further leads to translational repression or mRNA degradation. Recently, miR‑138 has been found to have a tumor suppressive role in a variety of human malignancies. However, the exact role of miR‑138 in regulating the malignant phenotypes of osteosarcoma (OS) has remained to be elucidated. In the present study, reverse‑transcription PCR analysis showed that the expression of miR‑138 was markedly reduced in OS tissues compared to that in matched adjacent non‑tumorous tissues. Furthermore, it was also downregulated in several common OS cell lines, when compared with that in a normal human osteoblast cell line. Overexpression of miR‑138 suppressed cell proliferation and invasion and led to a significant decrease in the protein expression of sirtuin 1 (SIRT1), which was further identified as a direct target gene of miR‑138 in MG63 cells. Moreover, restoration of SIRT1 expression reversed the suppressive effects of miR‑138 on MG63 cell proliferation and invasion. Finally, the expression of SIRT1 was found to be significantly upregulated in OS tissues compared to that in matched adjacent tissues, and SIRT1 levels were inversely correlated with the miR‑138 levels in OS tissues. Therefore, the present study demonstrated that miR‑138 has a role in inhibiting OS cell proliferation and invasion via directly targeting SIRT1, and suggested that the miR‑138/SIRT1 axis may become a promising therapeutic target for OS.

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June 2017
Volume 13 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
APA
Yuan, Z., Mo, H., Mo, L., He, J., Wu, Z., & Lin, X. (2017). Suppressive effect of microRNA-138 on the proliferation and invasion of osteosarcoma cells via targeting SIRT1. Experimental and Therapeutic Medicine, 13, 3417-3423. https://doi.org/10.3892/etm.2017.4426
MLA
Yuan, Z., Mo, H., Mo, L., He, J., Wu, Z., Lin, X."Suppressive effect of microRNA-138 on the proliferation and invasion of osteosarcoma cells via targeting SIRT1". Experimental and Therapeutic Medicine 13.6 (2017): 3417-3423.
Chicago
Yuan, Z., Mo, H., Mo, L., He, J., Wu, Z., Lin, X."Suppressive effect of microRNA-138 on the proliferation and invasion of osteosarcoma cells via targeting SIRT1". Experimental and Therapeutic Medicine 13, no. 6 (2017): 3417-3423. https://doi.org/10.3892/etm.2017.4426