IGF‑1 may predict the severity and outcome of patients with sepsis and be associated with microRNA‑1 level changes

Xu,Liang; Zhang,Weijun; Sun,Renhua; Liu,Jingquan; Hong,Jun; Li,Qian; Hu,Bangchuan; Gong,Fangxiao

doi:10.3892/etm.2017.4553

IGF‑1 may predict the severity and outcome of patients with sepsis and be associated with microRNA‑1 level changes

Authors:
- Liang Xu
- Weijun Zhang
- Renhua Sun
- Jingquan Liu
- Jun Hong
- Qian Li
- Bangchuan Hu
- Fangxiao Gong
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Affiliations: Department of Intensive Care Unit, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China, Department of Neurology, Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang 310006, P.R. China
Published online on: June 7, 2017 https://doi.org/10.3892/etm.2017.4553
Pages: 797-804

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Abstract

IGF-1 functions as an anti-oxidative stress molecule and some critical patients with sepsis have a lower level of serum IGF‑1. However, the association between IGF‑1 and the severity or prognosis of sepsis remains unclear. This study aimed to elucidate the relationship between serum IGF‑1 levels and the severity and prognosis of sepsis, and the possible mechanism was analyzed. Clinical characteristics of patients with sepsis were recorded and analyzed. Serum IGF‑1 levels and micro (mi)RNA‑1 levels were tested using radioimmunoassay and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis, respectively. The A549 cell line and HKC cell line were cultured in vitro and exposed to H2O2 with or without IGF‑1 treatment. Cell death was detected by analyzing cell death markers via ELISA kits, and miRNA‑1 levels were detected after H2O2 exposure using RT‑qPCR analysis. miRNA‑1 in cells was upregulated by transfection and IGF‑1 mRNA was detected to determine its relationship with miRNA‑1. Once again, cell ELISA kits were used to analyze cell death markers after transfection. Serum IGF‑1 levels were reduced in patients with sepsis, whereas miRNA‑1 levels were higher (P<0.05 vs. healthy control). Patients in the septic shock subgroup or dead patients had the lowest IGF‑1 levels and the highest miRNA‑1 levels (P<0.05 vs. sepsis and severe sepsis). IGF‑1 levels were inversely proportional to the miRNA‑1 level. In vitro, IGF‑1 reduced the cell death caused by H2O2. miRNA‑1 transfection effectively increased the sensitivity of cells to H2O2 damage by reducing the expression of IGF‑1, which was able to prevent cells from injury caused by H2O2. The transfection of negative control miRNA did not influence the level of IGF‑1 miRNA and the sensitivity to H2O2 damage. In conclusion, low IGF‑1 levels in patients with sepsis may predict increased severity of the condition and poor prognosis. The possible mechanism is that the excessive miRNA‑1 levels reduce IGF‑1 levels, resulting in insufficient anti‑oxidative action by IGF‑1 which increases the injury caused by oxidative stress in patients with sepsis.

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