Open Access

Analysis of differentially expressed genes in white blood cells isolated from patients with major burn injuries

  • Authors:
    • Gongjie Tang
    • Tao Zhang
    • Xinbo Wang
    • Zengmei Song
    • Fucun Liu
    • Qian Zhang
    • Ran Huo
  • View Affiliations

  • Published online on: August 7, 2017     https://doi.org/10.3892/etm.2017.4899
  • Pages: 2931-2936
  • Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to identify differentially expressed genes (DEGs) and their related functions and pathways of major burn injuries, and to prevent the occurrence of complications. The expression profiling of E-GEOD-37069 was downloaded from ArrayExpress Archive. The DEGs of major burn injuries were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Gene and Genomes (KEGG) functional enrichment analysis were then performed for the DEGs. Based on the KEGG database, a pathway relationship network was constructed, and DEGs in significant GO terms and pathways were investigated. Gene signal network and gene co-expression network of these inserted DEGs were constructed. A total of 3,328 DEGs of major burn injuries were identified, including 1,337 up‑ and 1,991 downregulated DEGs. These DEGs were mainly enriched into various GO terms, including transcription, DNA-dependent, signal transduction and blood coagulation. Moreover, they were also enriched into different pathways, such as hematopoietic cell lineage, metabolic pathway and chemokine signaling pathway. The pathway relationship network was constructed with 72 nodes. The MAPK signaling pathway was the hub node. Based on the same gene symbol, 702 DEGs were obtained, identified in both GO terms and pathways. Finally, the gene signaling network and gene co-expression network were constructed with 391 and 128 nodes, respectively. These identified DEGs, including GNB2, LILRA2, ARRB2 and ARHGEF2, may be potential key genes involved in the treatment of major burn injuries and prevention of complications.

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October-2017
Volume 14 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Tang G, Zhang T, Wang X, Song Z, Liu F, Zhang Q and Huo R: Analysis of differentially expressed genes in white blood cells isolated from patients with major burn injuries. Exp Ther Med 14: 2931-2936, 2017.
APA
Tang, G., Zhang, T., Wang, X., Song, Z., Liu, F., Zhang, Q., & Huo, R. (2017). Analysis of differentially expressed genes in white blood cells isolated from patients with major burn injuries. Experimental and Therapeutic Medicine, 14, 2931-2936. https://doi.org/10.3892/etm.2017.4899
MLA
Tang, G., Zhang, T., Wang, X., Song, Z., Liu, F., Zhang, Q., Huo, R."Analysis of differentially expressed genes in white blood cells isolated from patients with major burn injuries". Experimental and Therapeutic Medicine 14.4 (2017): 2931-2936.
Chicago
Tang, G., Zhang, T., Wang, X., Song, Z., Liu, F., Zhang, Q., Huo, R."Analysis of differentially expressed genes in white blood cells isolated from patients with major burn injuries". Experimental and Therapeutic Medicine 14, no. 4 (2017): 2931-2936. https://doi.org/10.3892/etm.2017.4899