Open Access

Wnt6 influences the viability of mouse embryonic palatal mesenchymal cells via the β‑catenin pathway

  • Authors:
    • Zheng Jiang
    • Lin Pan
    • Xiaoling Chen
    • Zhiqun Chen
    • Dongwei Xu
  • View Affiliations

  • Published online on: October 2, 2017     https://doi.org/10.3892/etm.2017.5240
  • Pages:5339-5344
  • Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The embryological stages of palatal shelf elongation and elevation, mainly induced by the proliferation and extracellular matrix secretion of embryonic palatal mesenchymal (MEPM) cells, are essential for normal palatal development. Wingless‑related MMTV integration site gene family (Wnt) signaling pathways serve key roles in craniofacial development and palate formation. Recent studies have indicated that Wnt6 participates in embryonic development of the palate, though its exact role in palate development remains unclear. In the present study, to investigate the role of Wnt6 during the stages of palatal shelves elongation and elevation, mouse MEPM cells were cultured from dissected palatal shelves at embryonic day 13.5. Results of an MTT assay and flow cytometric analysis demonstrated that treatment with recombinant Wnt6 increased the viability of MEPM cells (P<0.01) and the proportion of cells in the S and G2/M phases (P<0.01). Meanwhile, Wnt6 activated the β‑catenin signaling pathway as indicated by the dual luciferase assay result, and blockade of the WNT/β‑catenin pathway reduced the cytoactivity of Wnt6 in MEPM cells (P<0.01). Collectively, these findings indicate that Wnt6 promotes the vitality of MEPM cells by increasing the S + G2/M‑phase cell population, potentially through activation of the β‑catenin pathway during palatal shelf elongation and elevation.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Jiang, Z., Pan, L., Chen, X., Chen, Z., & Xu, D. (2017). Wnt6 influences the viability of mouse embryonic palatal mesenchymal cells via the β‑catenin pathway. Experimental and Therapeutic Medicine, 14, 5339-5344. https://doi.org/10.3892/etm.2017.5240
MLA
Jiang, Z., Pan, L., Chen, X., Chen, Z., Xu, D."Wnt6 influences the viability of mouse embryonic palatal mesenchymal cells via the β‑catenin pathway". Experimental and Therapeutic Medicine 14.6 (2017): 5339-5344.
Chicago
Jiang, Z., Pan, L., Chen, X., Chen, Z., Xu, D."Wnt6 influences the viability of mouse embryonic palatal mesenchymal cells via the β‑catenin pathway". Experimental and Therapeutic Medicine 14, no. 6 (2017): 5339-5344. https://doi.org/10.3892/etm.2017.5240