Open Access

MicroRNA-185 suppresses pancreatic cell proliferation by targeting transcriptional coactivator with PDZ‑binding motif in pancreatic cancer

  • Authors:
    • Di Xia
    • Xiaoyu Li
    • Qinghui Niu
    • Xishuang Liu
    • Wanqun Xu
    • Chengtai Ma
    • Huali Gu
    • Zhenfang Liu
    • Lei Shi
    • Xintao Tian
    • Xiaoxue Chen
    • Yubao Zhang
  • View Affiliations

  • Published online on: November 6, 2017     https://doi.org/10.3892/etm.2017.5447
  • Pages:657-666
  • Copyright: © Xia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to compare the expression of transcriptional coactivator with the PDZ‑binding motif (TAZ) in pancreatic cancer (PC) patients, and to investigate the regulation mechanisms of TAZ in the proliferation of PC. PC tissues and matched peritumoral tissues, pancreatic juice and serum were collected from PC patients who underwent pancreatectomy between June 2012 and December 2015 at the Affiliated Hospital of Qingdao University (Qingdao, China). Pancreatic juice and serum were collected from patients with chronic pancreatitis as a control. The levels of taz mRNA expression in the samples were examined by reverse‑transcription quantitative polymerase chain reaction, and the protein expression of TAZ was assessed by western blot analysis and ELISA. MicroRNAs (miRNAs) that regulate TAZ expression were also predicted by bioinformatics analysis and validated by dual luciferase reporter and rescue assays. In addition, the proliferation of PC cells was evaluated after transfection with TAZ small interfering RNA (siRNA) or its upstream miRNA agomir. Expression of TAZ was significantly increased in the PC tissues, pancreatic juice and serum of PC patients at the mRNA and protein levels compared with controls (P<0.05). Furthermore, TAZ was predicted and verified to be a target of miRNA (miR)‑185, and miR‑185 and TAZ were inversely expressed in samples from PC patients (P<0.05). In addition, TAZ siRNA or agomiR‑185 transfection significantly inhibited human pancreatic adenocarcinoma cell proliferation (P<0.05). However, overexpression of TAZ in the agomiR‑185 group rescued the inhibition (P<0.05). Finally, the expression of TAZ effector proteins, namely ankyrin repeat domain‑containing protein and cysteine‑rich 61, were upregulated in PC tissues (P<0.05), but repressed following transfection of PC cells with agomiR‑185 (P<0.05). Thus, miR‑185 may regulate the proliferation of PC by targeting TAZ, making it a promising diagnostic marker for PC.

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January 2018
Volume 15 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
(total number of cites)

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Copy and paste a formatted citation
APA
Xia, D., Li, X., Niu, Q., Liu, X., Xu, W., Ma, C. ... Zhang, Y. (2018). MicroRNA-185 suppresses pancreatic cell proliferation by targeting transcriptional coactivator with PDZ‑binding motif in pancreatic cancer. Experimental and Therapeutic Medicine, 15, 657-666. https://doi.org/10.3892/etm.2017.5447
MLA
Xia, D., Li, X., Niu, Q., Liu, X., Xu, W., Ma, C., Gu, H., Liu, Z., Shi, L., Tian, X., Chen, X., Zhang, Y."MicroRNA-185 suppresses pancreatic cell proliferation by targeting transcriptional coactivator with PDZ‑binding motif in pancreatic cancer". Experimental and Therapeutic Medicine 15.1 (2018): 657-666.
Chicago
Xia, D., Li, X., Niu, Q., Liu, X., Xu, W., Ma, C., Gu, H., Liu, Z., Shi, L., Tian, X., Chen, X., Zhang, Y."MicroRNA-185 suppresses pancreatic cell proliferation by targeting transcriptional coactivator with PDZ‑binding motif in pancreatic cancer". Experimental and Therapeutic Medicine 15, no. 1 (2018): 657-666. https://doi.org/10.3892/etm.2017.5447