Open Access

Evaluation of the inactivation effect of riboflavin photochemical method on duck hepatitis B virus

  • Authors:
    • Zi-Yu Zhou
    • Xing-Xiu Bi
  • View Affiliations

  • Published online on: November 13, 2017     https://doi.org/10.3892/etm.2017.5507
  • Pages:751-754
  • Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hepatitis B virus (HBV) is a common and widespread infection that poses a serious threat among carriers for the development of life-threatening liver diseases. The aim of the present study was to evaluate the effectiveness of the riboflavin photochemical method in inactivating duck hepatitis B virus (DHBV) in plasma via an animal model. Forty ducks were selected and randomly divided into the experimental (n=10), the virus control (n=10), the visible light control (n=10) and the plasma control group (n=10). Ducks in the experimental group were injected with plasma inactivated by the riboflavin photochemical method; in the virus control group were injected with plasma without inactivation treatment; in the visible light control group were injected with plasma irradiated by visible light; and in the plasma control group were injected with normal plasma. The serum of the ducks in each group was taken at different time points to detect DHBV-DNA levels via FQ-PCR and duck hepatitis B surface antigen (DHBsAg) via ELISA. DHBV-DNA in the experimental group was decreased gradually over time until it disappeared and there was a significant difference in DHBsAg between the experimental and control groups (P<0.05). In conclusion, the results showed that the riboflavin photochemical method is effective in the inactivation of viruses in plasma, which has relevance for preventive strategies against transfusion-derived infections.

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Journal Cover

January 2018
Volume 15 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Zhou, Z., & Zhou, Z. (2018). Evaluation of the inactivation effect of riboflavin photochemical method on duck hepatitis B virus. Experimental and Therapeutic Medicine, 15, 751-754. https://doi.org/10.3892/etm.2017.5507
MLA
Zhou, Z., Bi, X."Evaluation of the inactivation effect of riboflavin photochemical method on duck hepatitis B virus". Experimental and Therapeutic Medicine 15.1 (2018): 751-754.
Chicago
Zhou, Z., Bi, X."Evaluation of the inactivation effect of riboflavin photochemical method on duck hepatitis B virus". Experimental and Therapeutic Medicine 15, no. 1 (2018): 751-754. https://doi.org/10.3892/etm.2017.5507