Open Access

MicroRNA-383 upregulation protects against propofol-induced hippocampal neuron apoptosis and cognitive impairment

  • Authors:
    • Xinlei Wang
    • Guoyou Ding
    • Wei Lai
    • Shiwen Liu
    • Jun Shuai
  • View Affiliations

  • Published online on: February 5, 2018     https://doi.org/10.3892/etm.2018.5838
  • Pages: 3181-3188
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Anesthesia-induced cognitive impairment is a recognized clinical phenomenon. The present study aimed to investigate the effect of microRNA‑383 (miR‑383) expression on propofol‑induced learning and memory impairment. In total, 48 male Sprague‑Dawley rats (weight, 250±10 g) were randomly divided into four groups (n=12 each): Control group, and three groups of rats that were anesthetized with propofol for 6 h and untreated (propofol model group), treated with a constructed lentivirus vector expressing miR‑383 mimics (mimic + propofol group), or treated with miR‑383 scramble (scramble + propofol group). The learning memory ability, hippocampal neuron apoptosis and expression of apoptosis‑associated factors were detected using reverse transcription‑quantitiative polymerase chain reaction and western blot analysis. Propofol treatment significantly reduced the relative mRNA and protein expression of miR‑383, induced neuron apoptosis, upregulated the Bax/Bcl‑2 ratio, downregulated the relative mRNA and protein expression levels of postsynaptic density protein 95 and cAMP‑response element binding protein, and inactivated the phosphoinositide 3‑kinase/protein kinase B signaling pathway. By contrast, miR‑383 mimics significantly altered the propofol‑induced dysregulation of the aforementioned factors. In conclusion, miR‑383 mimic was able to repair propofol‑induced cognitive impairment via protecting against hippocampal neuron apoptosis and dysregulation of related factors. The present study suggested that miR‑383 may be used as a potential therapeutic target for the clinical treatment of cognitive impairment induced by propofol anesthesia.
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April-2018
Volume 15 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Wang X, Ding G, Lai W, Liu S and Shuai J: MicroRNA-383 upregulation protects against propofol-induced hippocampal neuron apoptosis and cognitive impairment. Exp Ther Med 15: 3181-3188, 2018.
APA
Wang, X., Ding, G., Lai, W., Liu, S., & Shuai, J. (2018). MicroRNA-383 upregulation protects against propofol-induced hippocampal neuron apoptosis and cognitive impairment. Experimental and Therapeutic Medicine, 15, 3181-3188. https://doi.org/10.3892/etm.2018.5838
MLA
Wang, X., Ding, G., Lai, W., Liu, S., Shuai, J."MicroRNA-383 upregulation protects against propofol-induced hippocampal neuron apoptosis and cognitive impairment". Experimental and Therapeutic Medicine 15.4 (2018): 3181-3188.
Chicago
Wang, X., Ding, G., Lai, W., Liu, S., Shuai, J."MicroRNA-383 upregulation protects against propofol-induced hippocampal neuron apoptosis and cognitive impairment". Experimental and Therapeutic Medicine 15, no. 4 (2018): 3181-3188. https://doi.org/10.3892/etm.2018.5838