Extract of Rhus verniciflua stokes protects against renal ischemia-reperfusion injury by enhancing Nrf2-mediated induction of antioxidant enzymes

Choi,Du  Ri; Jeong,Ji  Heun; Yu,Kwang‑Sik; Lee,Nam‑Seob; Jeong,Young‑Gil; Kim,Do  Kyung; Na,Chun  Soo; Na,Dae  Seung; Hwang,Won  Min; Han,Seung‑Yun

doi:10.3892/etm.2018.5913

Extract of Rhus verniciflua stokes protects against renal ischemia-reperfusion injury by enhancing Nrf2-mediated induction of antioxidant enzymes

Authors:
- Du Ri Choi
- Ji Heun Jeong
- Kwang‑Sik Yu
- Nam‑Seob Lee
- Young‑Gil Jeong
- Do Kyung Kim
- Chun Soo Na
- Dae Seung Na
- Won Min Hwang
- Seung‑Yun Han
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Affiliations: Department of Anatomy, College of Medicine, Konyang University, Daejeon 302‑718, Republic of South Korea, Lifetree Biotechnology Institute, Lifetree Biotech Co. Ltd, Suwon 441‑813, Republic of South Korea, Division of Nephrology, Department of Internal Medicine, Konyang University Hospital, Daejeon 302‑718, Republic of South Korea
Published online on: February 28, 2018 https://doi.org/10.3892/etm.2018.5913
Pages: 3827-3835
Copyright: © Choi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ischemia-reperfusion injury (IRI) may cause acute kidney disease (AKD) by mediating the oxidative stress-induced apoptosis of parenchymal cells. The extract of Rhus verniciflua Stokes (RVS) is used as a traditional herbal medicine as it exhibits anti‑oxidant, anti‑apoptotic and anti‑inflammatory properties. Therefore, the current study investigated the therapeutic effect and the underlying mechanism of RVS on IRI‑induced AKD in vivo and in vitro. The current study assessed the effects of RVS on a mouse model of renal IRI and in hypoxic human renal tubular epithelial HK‑2 cells. The results demonstrated that the IRI‑induced elevation of blood urea nitrogen, serum creatinine and lactate dehydrogenase was significantly attenuated by the intraoral administration of RVS (20 mg/kg/day) for 14 days prior to surgery. It was demonstrated that IRI surgery induced histological damage and cellular apoptosis in renal parenchyma, which were attenuated by pretreatment with RVS. Furthermore, in HK‑2 cells incubated with 300 µM CoCl2 to induce chemical hypoxia, it was demonstrated that RVS treatment significantly inhibited cell death and the production of reactive oxygen species (ROS). Furthermore, RVS treatment upregulated the levels of endogenous antioxidant enzymes, including heme oxygenase‑1 and catalase, as well as their upstream regulator nuclear factor erythroid 2‑related factor 2, in HK‑2 cells. Taken together, these results suggested that the intraoral administration of RVS induces a therapeutic effect on IRI‑induced AKD. These effects are at least partly due to the attenuation of ROS production via upregulation of the antioxidant defense system in renal tubular cells.

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