The clinical significance of Mda‑7/IL‑24 and C‑myb expression in tumor tissues of patients with diffuse large B cell lymphoma

Ma,Ming; Zhao,Riyang; Yang,Xingxiao; Zhao,Lianmei; Liu,Lihua; Zhang,Cong; Wang,Xuexiao; Shan,Baoen

doi:10.3892/etm.2018.6230

The clinical significance of Mda‑7/IL‑24 and C‑myb expression in tumor tissues of patients with diffuse large B cell lymphoma

Authors:
- Ming Ma
- Riyang Zhao
- Xingxiao Yang
- Lianmei Zhao
- Lihua Liu
- Cong Zhang
- Xuexiao Wang
- Baoen Shan
View Affiliations

Affiliations: Clinical Laboratory, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, Department of Infection Management, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, Department of Biotherapy, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China
Published online on: May 29, 2018 https://doi.org/10.3892/etm.2018.6230
Pages: 649-656
Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Diffuse large B cell lymphoma (DLBCL) is the most common type of non‑Hodgkin's lymphoma in adults. Mda‑7/IL‑24 had been identified as a differentiation inducer of B phenotype lymphoma cells. Previous studies have revealed that knockdown of C‑myb also leads to the terminal differentiation of B cell lymphoma. The aim of the present study was to investigate the association between the expression of Mda‑7/IL‑24 and C‑myb, and their prognostic significance for DLBCL patients. The tumor tissues were collected from 72 cases of DLBCL patients and detected with reverse transcription‑quantitative polymerase chain reaction, western blotting and immunohistochemistry assays. The results showed that, the expression of Mda‑7/IL‑24 mRNA and protein was lower while the expression of C‑myb was higher in DLBCL tissues, compared with the specimens of normal lymph node tissues. Furthermore, C‑myb expression was negatively correlated with Mda‑7/IL‑24 expression at mRNA and protein levels in DLBCL tissues. The expression of Mda‑7/IL‑24 and C‑myb in DLBCL tissues was associated with some clinicopathological parameters such as clinical stage, infiltration in bone marrow, Ki67 expression level in the tumor tissues and overall survival rates. These results indicated that low expression of Mda‑7/IL‑24, along with high expression of C‑myb, are predictor for poor prognosis of DLBCL patients, suggesting that Mda‑7/IL‑24 and C‑myb may be potential targets for clinical treatment of DLBCL.

View Figures

View References

1	Li J, Fu R, Yang L and Tu W: miR-21 expression predicts prognosis in diffuse large B-cell lymphoma. Int J Clin Exp Pathol. 8:15019–15024. 2015.PubMed/NCBI
2	Ma M, Zhao L, Sun G, Zhang C, Liu L, Du Y, Yang X and Shan BE: Mda-7/IL-24 enhances sensitivity of B cell lymphoma to chemotherapy drugs. Oncol Rep. 35:3122–3130. 2016. View Article : Google Scholar : PubMed/NCBI
3	Yang BX, Duan YJ, Dong CY, Zhang F, Gao WF, Cui XY, Lin YM and Ma XT: Novel functions for mda-7/IL-24 and IL-24 delE5: Regulation of differentiation of acute myeloid leukemic cells. Mol Cancer Ther. 10:615–625. 2011. View Article : Google Scholar : PubMed/NCBI
4	Dong CY, Zhang F, Duan YJ, Yang BX, Lin YM and Ma XT: mda-7/IL-24 inhibits the proliferation of hematopoietic malignancies in vitro and in vivo. Exp Hematol. 36:938–946. 2008. View Article : Google Scholar : PubMed/NCBI
5	Hosseini E, Hosseini SY, Hashempour T, Fattahi MR and Sadeghizadeh M: Effect of RGD coupled MDA-7/IL-24 on apoptosis induction in a hepatocellular carcinoma cell line. Mol Med Rep. 15:495–501. 2017. View Article : Google Scholar : PubMed/NCBI
6	Huo W, Li ZM, Zhu XM, Bao YM and An LJ: MDA-7/IL-24 suppresses tumor adhesion and invasive potential in hepatocellular carcinoma cell lines. Oncol Rep. 30:986–992. 2013. View Article : Google Scholar : PubMed/NCBI
7	Ma M, Yang X, Zhao L, Liu L, Zhang C, Wang X and Shan B: Mda-7/IL-24 induces the differentiation of B cell lymphoma via activation of the P38 mitogen activated protein kinase signaling pathway. Mol Med Rep. 16:5633–5642. 2017. View Article : Google Scholar : PubMed/NCBI
8	Liu X, Zhou H, Huang X, Cui J, Long T, Xu Y, Liu H, Yu R, Zhao R, Luo G, et al: A broad blockade of signaling from the IL-20 family of cytokines potently attenuates collagen-induced arthritis. J Immunol. 197:3029–3037. 2016. View Article : Google Scholar : PubMed/NCBI
9	Panneerselvam J, Srivastava A, Muralidharan R, Wang Q, Zheng W, Zhao L, Chen A, Zhao YD, Munshi A and Ramesh R: IL-24 modulates the high mobility group (HMG) A1/miR222/AKT signaling in lung cancer cells. Oncotarget. 7:70247–70263. 2016. View Article : Google Scholar : PubMed/NCBI
10	Piovesan D, Tempany J, Di Pietro A, Baas I, Yiannis C, O'Donnell K, Chen Y, Peperzak V, Belz GT, Mackay CR, et al: c-Myb regulates the T-bet-dependent differentiation program in B cells to coordinate antibody responses. Cell Rep. 19:461–470. 2017. View Article : Google Scholar : PubMed/NCBI
11	Iżykowska K, Przybylski GK, Gand C, Braun FC, Grabarczyk P, Kuss AW, Olek-Hrab K, Bastidas Torres AN, Vermeer MH, Zoutman WH, et al: Genetic rearrangements result in altered gene expression and novel fusion transcripts in Sézary syndrome. Oncotarget. 8:39627–39639. 2017. View Article : Google Scholar : PubMed/NCBI
12	Jaffe ES: The 2008 WHO classication of lymphomas: Implications for clinical practice and translational research. Hematology Am Soc Hematol Educ Program. pp. 523–531. 2009, PubMed/NCBI
13	Zelenetz AD, Gordon LI, Wierda WG, Abramson JS, Advani RH, Andreadis CB, Bartlett N, Byrd JC, Czuczman MS, Fayad LE, et al: Non-Hodgkin's lymphomas, version 4.2014. J Natl Compr Canc Netw. 12:1282–1303. 2014. View Article : Google Scholar : PubMed/NCBI
14	Xu SL, Oshima T, Imada T, Musuda M, Debnath B, Grande F, Garofalo A and Neamati N: Stabilization of MDA-7/IL-24 for colon cancer therapy. Cancer Lett. 335:421–430. 2013. View Article : Google Scholar : PubMed/NCBI
15	Chen S, Wang Z, Dai X, Pan J, Ge J, Han X, Wu Z, Zhou X and Zhao T: Re-expression of microRNA-150 induces EBV-positive Burkitt lymphoma differentiation by modulating c-Myb in vitro. Cancer Sci. 104:826–834. 2013. View Article : Google Scholar : PubMed/NCBI
16	Cheng HR, Wu BQ, Chen L, Zhang ZX and Li B: Expression and effect of serum interleukin-24 level on bone marrow mononuclear cells in children with acute leukemia. Genet Mol Res. 14:17281–17288. 2015. View Article : Google Scholar : PubMed/NCBI
17	Patani N, Douglas-Jones A, Mansel R, Jiang W and Mokbel K: Tumour suppressor function of MDA-7/IL-24 in human breast cancer. Cancer Cell Int. 10:292010.PubMed/NCBI
18	Galimova E, Rätsep R, Traks T, Kinggo K, Escott-price V and Kõks S: Interleukin-10 family cytokines pathway: Genetic variants and psoriasis. Br J Dermatol. 176:1577–1587. 2017. View Article : Google Scholar : PubMed/NCBI
19	Sang M, Hulsurkar M, Zhang X, Song H, Zheng D, Zhang Y, Xu J, Zhang S, Ittmann M and Li W: GRK3 is a direct target of CREB activation and regulates neuroendocrine differentiation of prostate cancer cells. Oncotarget. 7:45171–45185. 2016. View Article : Google Scholar : PubMed/NCBI
20	Xia Y, Xu-Monette ZY, Tzankov A, Li X, Manyam GC, Murty V, Bhagat G, Zhang S, Pasqualucci L, Visco C, et al: Loss of PRDM1/BLIMP-1 function contributes to poor prognosis of activated B-cell-like diffuse large B-cell lymphoma. Leukemia. 31:625–636. 2017. View Article : Google Scholar : PubMed/NCBI
21	Chan YH, Chiang MF, Tsai YC, Su ST, Chen MH, Hou MS and Lin KI: Absence of the transcriptional repressor Blimp-1 in hematopoietic lineages reveals its role in dendritic cell homeostatic development and function. J Immunol. 183:7039–7046. 2009. View Article : Google Scholar : PubMed/NCBI
22	Pradhan AK, Talukdar S, Bhoopathi P, Shen XN, Emdad L, Das SK, Sarkar D and Fisher PB: Mda-7/IL-24 mediates cancer cell-specific death via regulation of miR-221 and the beclin-1 axis. Cancer Res. 77:949–959. 2017. View Article : Google Scholar : PubMed/NCBI
23	Ma YF, Ren Y, Wu CJ, Zhao XH, Xu H, Wu DZ, Xu J, Zhang XL and Ji Y: Interleukin (IL)-24 transforms the tumor microenvironment and induces anticancer immunity in a murine model of colon cancer. Mol Immunol. 75:11–20. 2016. View Article : Google Scholar : PubMed/NCBI
24	Lv C, Su Q, Liang Y, Hu J and Yuan S: Oncolytic vaccine virus harbouring the IL-24 gene suppresses the growth of lung cancer by inducing apoptosis. Biochem Biophys Res Commun. 476:21–28. 2016. View Article : Google Scholar : PubMed/NCBI